We narrowed to 5,258 results for: pcDNA3.1+

1. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_GEM22-Halo-FRB

   Plasmid

   #197059

   Purpose

   Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_FRB-GEM23-Halo

   Plasmid

   #197060

   Purpose

   Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   I3-01
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-mPCSK3 H194L

   Plasmid

   #122689

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Histidine 194 to Leucine

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-mPCSK3 S368A

   Plasmid

   #122680

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Serine 368 to Alanine

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172314

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

   Depositor

   Joshua Rosenthal

   Article

   Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Use

   Tags

   6xHis, Flag, and Nuclear Localization Signal of S…

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-3xFLAG-RSL1D1(WT)

   Plasmid

   #122301

   Purpose

   expresses RSL1D1 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RSL1D1 (RSL1D1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

   Plasmid

   #148118

   Purpose

   Mammalian Expression of HshnRNPE2iso3

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPE2iso3 (PCBP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation L239 to S compapred to th…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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