We narrowed to 5,413 results for: PEP
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Plasmid#212901PurposeEncodes S.cerevisiae SUC2 (Invertase) pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD021
Plasmid#212907PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFapp8) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD024
Plasmid#212910PurposeEncodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD061
Plasmid#212915PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD022
Plasmid#212908PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD009
Plasmid#212906PurposeEncodes S. cerevisiae SRL1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSRL1 (SRL1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD100
Plasmid#212924PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-mTP-TurboID
Plasmid#209396PurposeTransiently expressing TurboID fused with a Mitochondrial transit peptide (mTP) in plantaDepositorInsertmTP-3XHA-TurboID
UseTagsExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-DIIG
Plasmid#201196PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-DIIG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-SWG
Plasmid#201194PurposeExpresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-SWG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-PEED
Plasmid#201195PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-PEED
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYUBcLIC-GFP
Plasmid#183509PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.DepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_1G11F2-3 heavy chain
Plasmid#198253Purposerecombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3DepositorInsertheavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
UseTagsIL10 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xHAtag-SP-CLSTN2
Plasmid#191694PurposeFor expression coding sequencing of CLSTN2DepositorInsertsignal peptide, 3xHAtag and full length coding sequence of CLSTN2
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC
Plasmid#192376PurposeTo test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.DepositorInsertAct2::FRT-uORF-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa1-654)
Plasmid#189558PurposeProtein expression ADAM10 in mammalian cells. Contains only prodomain through cysteine rich domain--amino acids 1-654DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa1-654PromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SP-3xHAtag-PTPRO
Plasmid#191695PurposeFor expression coding sequencing of PTPRODepositorInsertsignal peptide, full length coding sequence of PTPRO and 3xHAtag
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2393-Tier1-PhCMV-p2a_fastFT
Plasmid#169530PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, fast maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2392-Tier1-PhCMV-p2a_medFT
Plasmid#169529PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, medium maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2388-Tier1-PhCMV-p2a_Ypet
Plasmid#169525PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).DepositorInsertPCMV-driven P2A-fused yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2387-Tier1-PhCMV-p2a_mTagBFP2
Plasmid#169524PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).DepositorInsertPCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2389-Tier1-PhCMV-p2a_mRuby
Plasmid#169526PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).DepositorInsertPCMV-driven P2A-fused enhanced yellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-epELM-5
Plasmid#179391PurposeExpress a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteriaDepositorInsertELMSAN1 (MIDEAS Human)
UseTags6x HisExpressionBacterialMutationPromoterT7 lacAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-GHtag-HDEL
Plasmid#136096PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tagDepositorInsertCTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglA
Plasmid#170720PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)DepositorInsertRhamnogalacturonan lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-4
Plasmid#163130PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-PRGRH-Nluc
Plasmid#158608PurposePlasmid expressing PRG(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-mas-p115RH-Nluc
Plasmid#158607PurposePlasmid expressing p115(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCVpic2hygro-EGFP-FF2
Plasmid#164921PurposeExpresses EGFP cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-1
Plasmid#163137PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz174_CsoS2_N3_GFP
Plasmid#140854PurposeHis-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 3 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz26_CsoS2_N1-N2_GFP
Plasmid#140851PurposeHis-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backboneDepositorInsertCsoS2 NTD repeats 1-2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
D41_Ld_180450
Plasmid#135855PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertserine carboxypeptidase (CBP1), putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
D69_Ld_191460
Plasmid#135882PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertcysteine peptidase A (CPA)
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
D1_Ld_290860
Plasmid#135817PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertcysteine peptidase C (CPC)
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMACHis6_PiuD
Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
UseTags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-001428gp160-754*
Plasmid#123252PurposeMammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncationDepositorInsertHIV-1 (001428) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-AD8gp160-754*
Plasmid#123257PurposeMammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncationDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp160-754*
Plasmid#123248PurposeMammalian expression plasmid for Env from the DU422 HIV-1 isolate; C-terminal truncationDepositorInsertHIV-1 (DU422) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only