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pINDUCER20-Flag-NLS-hSPDEF delta3

Plasmid

#100876

Purpose

Lentiviral expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 243-335aa

Promoter

TRE2

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta3

Plasmid

#100872

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 244-335aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1-Flag-NLS-hSPDEF delta1

Plasmid

#100870

Purpose

Mammalian expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 1-248aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 N184X

Plasmid

#92373

Purpose

Inducible expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP S245X with 5'UTR

Plasmid

#92376

Purpose

Inducible expression of S245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 S245X

Plasmid

#92374

Purpose

Inducible expression of M87 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 S245X

Plasmid

#92372

Purpose

Inducible expression of M1S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 N184X

Plasmid

#92371

Purpose

Inducible expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/SP S245X with 5'UTR

Plasmid

#92370

Purpose

expression of 245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S245X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 N184X

Plasmid

#92367

Purpose

expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 N184X

Plasmid

#92365

Purpose

expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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Banshee-ShCit-A

Plasmid

#85216

Purpose

Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

shCit-A

Use

Retroviral

Expression

Mammalian

Promoter

H1

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Talin-ABM-10

Plasmid

#55510

Purpose

Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Talin ABM

Tags

mTFP1

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pET-20b-T7-Ntx

Plasmid

#58714

Purpose

Expresses T7-Notexin in Escherichia coli

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

T7 peptide tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mCherry

Plasmid

#62635

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pPPAT-2XStrep

Plasmid

#125620

Purpose

expresses human PPAT protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PPAT (PPAT Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJRoC30_Tv9nL

Plasmid

#188069

Purpose

PROSS-designed high-redox potential laccase from Trametes versicolor

Depositor

Sarel Fleishman

Article

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

PROSS-designed high-redox potential laccase from Trametes versicolor

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

79 PROSS mutations, described in publication

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-GEM-GECO1

Plasmid

#32442

Purpose

Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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