We narrowed to 2,493 results for: ERK

1. pGAL4-DBD-HEY2-Adel-IDR

   Plasmid

   #145234

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HEY2-Adel-IDR (HEY2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAL4-DBD-BHLHE41-Adel-IDR

   Plasmid

   #145228

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   BHLHE41-Adel-IDR (BHLHE41 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEHISSSO6206

   Plasmid

   #97065

   Purpose

   Expressing Sulfolobus solfataricus orf SSO6206 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus orf SSO6206
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-Puro-MEK-DD

   Plasmid

   #15268

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   Map2k1 (Mapk1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S218D S222D

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Promoter

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

   Plasmid

   #128605

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   24xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDECKO_GFP

   Plasmid

   #141210

   Purpose

   empty GFP vector for cloning sgRNA or pgRNAs

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLHCX-ATF4 mScarlet NLS

   Plasmid

   #115970

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC1-V16-Luc

    Plasmid

    #122034

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-MCS-MED1long-IDR-YFP-NLS

    Plasmid

    #145276

    Purpose

    Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MED1-IDR long (MED1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid

    #89463

    Purpose

    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Use

    Tags

    E. coli DHFRdd destabilization domain and mCherry…

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145270

    Purpose

    Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(53Q)-IDR (TBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145269

    Purpose

    Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(38Q)-IDR (TBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2K7bsdUBI-mCherry-STIM1

    Plasmid

    #114178

    Purpose

    Lentiviral vector for expression of mCherry-STIM1

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry (inserted after signal peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYX233-b2-DHFR

    Plasmid

    #163759

    Purpose

    Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    b2-DHFR (Dhfr Budding Yeast, Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

    Promoter

    GAL1

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

    Plasmid

    #65446

    Purpose

    Expression of ephrinB1 and RFP in mammalian cells

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    ephrin B1 (Efnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    SV40-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBQ

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pENTR1a-mCherry-STIM1

    Plasmid

    #114176

    Purpose

    Gateway entry clone containing mCherry-STIM1

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Gateway entry clone

    Tags

    mCherry (inserted after signal peptide)

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. S10A mutant of H3S10ph biosensor

    Plasmid

    #120810

    Purpose

    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 10 is mutated to Alanine, and Threonine 3,…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDECKO_GFP_AAVS1

    Plasmid

    #141211

    Purpose

    pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge