We narrowed to 165,214 results for: Gene

1. pHL-H1-ccdB-mEF1a-RiH

   Plasmid

   #60601

   Purpose

   Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   Red Fluorescent Protein
   Hygromycin resistance gene
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Donor_ANKRD1_KO_Puro

   Plasmid

   #186667

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Puromycin (ANKRD1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Donor_YTHDF2_KO_Hygro

   Plasmid

   #186671

   Purpose

   Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Hygromycin (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor_YTHDF2_KO_Puro

   Plasmid

   #186670

   Purpose

   Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Puromycin (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

   Plasmid

   #176059

   Purpose

   Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R124A and K125A

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry

   Plasmid

   #179431

   Purpose

   Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-coreHS4-mch (SC)
   

   Expression

   Mammalian

   Promoter

   pEF alpha, pRSV

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 (-) hYAP 1-1delta

   Plasmid

   #124142

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Marius Sudol, Megan Finch-Edmondson

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 (-) hYAP 1-2gamma

   Plasmid

   #124145

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Marius Sudol, Megan Finch-Edmondson

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Flag-Avi-ires-CHD5

    Plasmid

    #68878

    Purpose

    Transient mammalian expression of CHD5 for BirA tagging

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG/AVI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 (-) hYAP 1-1alpha

    Plasmid

    #124139

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLY54

    Plasmid

    #130923

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE (hygro) hYAP 1-1delta

    Plasmid

    #124150

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-GST-CHD5(Chromos)-FRAG

    Plasmid

    #68875

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only the two chromodomains of CHD5

    Promoter

    tac

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1 (-) hYAP 1-2beta

    Plasmid

    #124144

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 (-) hYAP 1-1gamma

    Plasmid

    #124141

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE (hygro) mEOS2 hYAP 1-2delta

    Plasmid

    #124162

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-FLAG-CHD5-Y619E-FRAG

    Plasmid

    #68871

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa, Y619E

    Promoter

    CMV

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-FLAG-CHD5-WT-331-672aa

    Plasmid

    #68870

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa)

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa

    Promoter

    CMV

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid

    #235303

    Purpose

    Lentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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