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pDSAP

Plasmid

#62293

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAY

Plasmid

#62291

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYOY153

Plasmid

#23107

Depositor

Linda Wordeman

Article

Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Mutation

H530-A, R534-A, K537-A

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCsn2

Plasmid

#113251

Purpose

E. coli expression vector for S. pyogenes Csn2-MBP-His

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Csn2

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas2

Plasmid

#113249

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas2

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas2

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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GGDestX1-Amp

Plasmid

#157652

Purpose

Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Cell-free expression (e. coli sigma-70)

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDSAG

Plasmid

#62289

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAT

Plasmid

#62290

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pNOC-NLUC

Plasmid

#98141

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pattB-RfB2

Plasmid

#62296

Purpose

docking site transgenesis plasmid for Gateway Cloning and insect transgenesis

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Unspecified

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAYN

Plasmid

#62294

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRB326

Plasmid

#8667

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Use

Centromeric

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pCAGGS mutated Pdx1 (DM#233)

Plasmid

#15072

Depositor

Douglas Melton

Article

Grapin-Botton et al Genes Dev. 2001 Feb 15. 15(4):444-54.

Insert

Pdx1

Expression

Mammalian

Mutation

DNA binding mutant

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pGLU_119

Plasmid

#225799

Purpose

RS421 library variant 14434 in pGLDV_18 (p15A)

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

14434_[Tn5]

Use

Synthetic Biology

Mutation

N/A

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGLU_83

Plasmid

#225784

Purpose

RS421 library variant 14434 in IDT backbone

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

14434_[abr]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSN859

Plasmid

#190692

Purpose

Mammalian expression of kinesin scFv(H2) fused with Fc domain of chicken IgY

Depositor

Shinsuke Niwa

Article

Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

Insert

kinesin scFv(H2)::Fc domain of chicken IgY

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC-FLUC

Plasmid

#98140

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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UB13p::dsSPL10 v01 (SPL10-RNAi)

Plasmid

#27409

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 inverted (AT1G27370 Mustard Weed)

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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