We narrowed to 4,195 results for: spl
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Plasmid#188263PurposePlasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnERb446 (ESR1 Human)
ExpressionMammalianAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
nTRb412-gs10-NLS-VPR
Plasmid#188267PurposePlasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnTRb412 (TXNRD2 Human)
ExpressionMammalianAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human-4xUAS
Plasmid#125150PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC1825_pCR8-dCasRx-RBM38
Plasmid#118662PurposeGateway Donor; dCasRx-RBM38 ORF; No promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCasRx-RBM38
UseCRISPRExpressionMammalianPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_NTD1-80_E17R
Plasmid#104479Purposebacterial expression of TDP-43 NTD E17RDepositorAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta
Plasmid#79913PurposepiggyBac transposon vector, Zeocin-2A-sHRPaNRX3betaDepositorInsertZeocin-t2A-sHRPbNRX3beta
UsePiggybacExpressionMammalianAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCB320-sgNC.SPA
Plasmid#169834PurposeExpresses a negative control sgRNADepositorInsertsafe harbor sgRNA
UseCRISPR, Lentiviral, and Mouse TargetingExpressionMammalianPromotermU6Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
ExpressionBacterialPromoterPBADAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc090
Plasmid#161937PurposeCarries engineered transposon for IBMDepositorInsertpTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
ExpressionBacterialAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Desmin/pAS2-1
Plasmid#97407PurposeDesmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertDesmin (DES Human)
ExpressionYeastAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)MutationCodon optimizedAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2 Cryptic PE-Max
Plasmid#216163PurposeExpression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor CommentsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEMax for prime editing with internal cryptic exon
UseCRISPRExpressionMammalianAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS158
Plasmid#215679PurposeEmpty repair plasmid for ChrIII split hygromycinR landing padDepositorInsert5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxExpressionWormMutation5' ∆HYGR encodes aa1-226Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Dest-loxP-sfGFP
Plasmid#202715PurposeEmpty expression vector containing Cre recombinase reporter.DepositorInsertPw7-loxP-term-loxP-sfGFP
UseCre/LoxExpressionBacterialPromoterconstitutiveAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)
Plasmid#172066PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intronDepositorInsertmTagBFP2(11x7)
ExpressionInsectAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11
Plasmid#172068PurposeProtein trap plasmid for inserting GFP11 into a phase 1 coding intronDepositorInsertGFP11x7
ExpressionInsectAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
P16ΔS:(MCS)-3xHA-S11
Plasmid#108185PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterP16ΔSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24-His6-GFP1-10
Plasmid#253053PurposeEncodes 6xHistidine-tagged GFP1-10 fluorescence complementation fragment in a modified pET24d vector backbone for expression in E. coliDepositorInsertGFP
ExpressionBacterialAvailable sinceMay 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS 3xFLAG Mbnl2
Plasmid#217032PurposeOverexpression of Mbnl2 in mammalian cellsDepositorInsertMbnl2 (Mbnl2 Mouse)
ExpressionMammalianAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC3
Plasmid#172996Purposeconstitutive expression of FLAG-tagged WWC3DepositorAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only