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pDONR223_DVL1_WT

Plasmid

#82110

Purpose

Gateway Donor vector containing DVL1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DVL1 (DVL1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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myc EDC4 pCMVSport6

Plasmid

#86855

Purpose

mammalian expression of EDC4

Depositor

Sarah Slavoff

Article

D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

Insert

EDC4 (EDC4 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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sFlt1-e15a V5

Plasmid

#86045

Purpose

human Soluble Flt1 isoform e15a (terminates in exon 15a)

Depositor

Christie Thomas

Article

Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

Insert

human soluble Flt1 isoform e15a (FLT1 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

isoform e15a (terminates in exon 15a)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-C12orf66

Plasmid

#87047

Purpose

mammalian expression of C12orf66

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

C12orf66 (C12orf66 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

Plasmid

#85070

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Tags

IRES-sYFP2-PH and mScarlet-H

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYAA

Plasmid

#80756

Purpose

Expression of human alpha A-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYAA (CRYAA Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nHA Spinophilin

Plasmid

#87122

Purpose

mammalian expression of Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-AMPH1-BAR-mCherry

Plasmid

#85130

Purpose

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcsII-pb4-flag

Plasmid

#86768

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 4 (PSMB4 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLXSN-Axl DN

Plasmid

#65223

Purpose

Expression of dominant negative Axl in mammalian cells

Depositor

Axel Ullrich

Article

Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

Insert

AXL DN (AXL Human)

Use

Retroviral

Expression

Mammalian

Mutation

deleted cytoplasmic domain

Promoter

LTR

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only