159,332 results

1. pMSP1E2D1

   Plasmid

   #161606

   Purpose

   Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009 . 464():211-31.

   Insert

   MSP1E2D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LAMP1-mRFP-FLAG

   Plasmid

   #34611

   Purpose

   3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

   Depositor

   David Sabatini

   Article

   Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   2xFlag and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC

   Plasmid

   #221365

   Purpose

   Mammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Rho IPI-[1D4] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTipi2.1_Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa

   Plasmid

   #221366

   Purpose

   Mammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. EGFP-p300

   Plasmid

   #191764

   Purpose

   Expression of EGFP fused to core p300 histone acetyltransferase

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (EP300 Human)
   

   Use

   Tags

   EGFP (N-terminal of p300 core) and FRB (N-termina…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-mGFP

   Plasmid

   #14757

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Has Service

   Cloning Grade DNA

   Insert

   membrane bound form of EGFP
   

   Use

   Tags

   Palmitoylation signal

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-GGG-ELP(40nm)-Cys

   Plasmid

   #91571

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(40nm)-Cys
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-OSBP-PH

   Plasmid

   #49571

   Purpose

   expresses the plekstrin homology domain of OSBP in mammalian cells

   Depositor

   Marci Scidmore

   Article

   Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

   Insert

   OSBP-PH (AA 91-179) (OSBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLIK-Hygro

   Plasmid

   #25737

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid

    #190236

    Purpose

    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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