We narrowed to 165,221 results for: Gene

1. pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

   Plasmid

   #235306

   Purpose

   Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   EGFP-Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Donor_YTHDF2_KO_Neo

   Plasmid

   #186672

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Neomycin (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Donor_ANKRD1_KO_Hygro

   Plasmid

   #186668

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Hygromycin (ANKRD1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9_ANKRD1_sgRNA2

   Plasmid

   #186669

   Purpose

   Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
   

   Expression

   Mammalian

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

   Plasmid

   #179433

   Purpose

   Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   coreHS4-TRE-cit-coreHS4-mch (DC)
   

   Expression

   Mammalian

   Promoter

   pEF alpha, pRSV

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

   Plasmid

   #179426

   Purpose

   Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-5kb-mch
   

   Expression

   Mammalian

   Promoter

   pEF alpha, pRSV

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry

   Plasmid

   #179427

   Purpose

   Dual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-1.2kb-mch
   

   Expression

   Mammalian

   Promoter

   pEF alpha, pRSV

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLY9

   Plasmid

   #130907

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-2G6, and Ptet

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY59

   Plasmid

   #130927

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   sfgfp
   pspFΔHTH::MCP
   

   Use

   Synthetic Biology

   Tags

   ASV tag and MCP (MS2 coat protein)

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-2G6, and Ptet

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLY55

    Plasmid

    #130924

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY53

    Plasmid

    #130911

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBABE (hygro) hYAP 1-2delta

    Plasmid

    #124154

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABE (hygro) mEOS2 hYAP 1-1alpha

    Plasmid

    #124155

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE (hygro)mEOS2 hYAP 1-1gamma

    Plasmid

    #124157

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBABE (hygro) mEOS2 hYAP 1-1delta

    Plasmid

    #124158

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE (hygro) mEOS2 hYAP 1-2alpha

    Plasmid

    #124159

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBABE (hygro) mEOS2 hYAP 1-2beta

    Plasmid

    #124160

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE (hygro) mEOS2 hYAP 1-2gamma

    Plasmid

    #124161

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge