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We narrowed to 1,747 results for: lin tian

Showing: 1701 - 1720 of 1747 results
  1. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

    Plasmid
    #182354
    Purpose
    Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid
    #182356
    Purpose
    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Tags
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pOD2045-intControl

    Plasmid
    #100005
    Purpose
    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).
    Depositor
    Inserts
    zif-1 (zif-1 Nematode)
    unc-119
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pOD2047-bwmControl

    Plasmid
    #100006
    Purpose
    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).
    Depositor
    Inserts
    zif-1 (zif-1 Nematode)
    unc-119
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

    Plasmid
    #182359
    Purpose
    Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.
    Insert
    Ultra-pan-neuronal positive control pUNP::gfp(NLS)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Promoter Ultra Pan Neuronal
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1701 - 1720 of 1747 results