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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-F14F15S-Switch

Plasmid

#99733

Purpose

Flp-based FLEX reporter (from RFP to GFP)

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

TagRFP and EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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hHS1-M7A,H102A

Plasmid

#159171

Purpose

Human codon-optimized cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

hHS1-M7A,H102A

Expression

Mammalian

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pME-FastFUCCI (JDW 1488)

Plasmid

#242556

Purpose

Gateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle state

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA-IRES-nls-EGFP

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pME-GcAMP5

Plasmid

#126569

Purpose

Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

GcAMP5

Use

Gateway entry clone

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGM32DEST

Plasmid

#74747

Purpose

Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

Gateway cassette
QF-GR (NR3C1 Synthetic, Human, Neurospora crassa)

Tags

Fusion of the Neurospora crassa QF activator to t…

Expression

Worm

Promoter

No promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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DRH322: AAV-CaMKIIa-QuasAr2-mO2

Plasmid

#51696

Purpose

AAV production of QuasAr2 fluorescent reporter

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

AAV

Tags

mOrange2

Promoter

CaMKIIa

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pJT311 AAV-Rapamycin Inducible-NZF-Citrine

Plasmid

#202060

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZF-Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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yHS1-M7A,H102A

Plasmid

#159152

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A,H102A

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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