168,341 results
-
Plasmid#21369DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
TagsFLAGExpressionMammalianMutationwild-type sequence*Available SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
Rosa26 mT/mG
Plasmid#17787DepositorInsertRosa26 mT/mG
UseCre/Lox and Mouse TargetingTagsmembrane tagExpressionMammalianAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJMP1
Plasmid#79873PurposeBacillus subtilis dCas9 expression vector; integrates into lacA/ganADepositorInsertdCas9
UseCRISPRExpressionBacterialMutationD10A, H840APromoterxylAAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-NCAPH
Plasmid#31214DepositorAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX MLKL(S345D)-2A-mCherry-puro
Plasmid#231975PurposeTet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosisDepositorInsertMLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)
UseLentiviralMutationMLKL S345DAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
GPR68-Tango
Plasmid#66371PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_OT1.0
Plasmid#185384PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cellsDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
ExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_EML4:1-981
Plasmid#211315PurposeMammalian expression of full-length human EML4. C-terminal HiBiT tag.DepositorInsertEML4:M1-S981 (EML4 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationI382V natural variant (VAR_031727)PromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only