164,765 results

1. pAAV-hChATp-tdTomato

   Plasmid

   #197564

   Purpose

   Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

   Depositor

   Casey Maguire

   Article

   Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human ChAT promoter

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-6HIS-TEV-mEGFP,IRES-NGFR

   Plasmid

   #164521

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AP4mS_PPVs_P3_cLuc

   Plasmid

   #119300

   Purpose

   Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBZCas13b

   Plasmid

   #89898

   Purpose

   Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 FLAG-YY1

   Plasmid

   #104396

   Purpose

   FLAG-YY1

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   YY1
   

   Use

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

   Plasmid

   #174081

   Purpose

   Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
   

   Use

   Tags

   NES-Flag

   Expression

   Mammalian

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   CMV

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19_HDRT_TRAC_CD19-CAR

   Plasmid

   #183473

   Purpose

   Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

   Depositor

   Dimitrios Wagner

   Article

   Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

   Inserts

   TRAC (TRAC Human)
   CD19-specific CAR transgene insert
   TRAC (TRAC Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT13araE

   Plasmid

   #18987

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

   Insert

   araE (araE E.coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-mCherry-CDK4KTR

    Plasmid

    #126680

    Purpose

    Fluorescent reporter for CDK4/6 activity

    Depositor

    Hee Won Yang

    Article

    Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

    Insert

    RB cterm
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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