We narrowed to 21,775 results for: Sis

1. pLC-mCherry-MLKL

   Plasmid

   #75167

   Purpose

   LentiCRISPR-mCherry with sgRNA targeting human MLKL

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   MLKL sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB1alpha2_SulR

   Plasmid

   #186425

   Purpose

   transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Insert

   SulR
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF005-sgRNA-shuffle-in

   Plasmid

   #104441

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF004-sgRNA-shuffle-in

   Plasmid

   #104440

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   U6 promoter

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-CIBN-EGFP-KRasCT

   Plasmid

   #178527

   Purpose

   Expresses CIBN-EGFP-KRasCT in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CIBN-EGFP-KRasCT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_Anc689-nCas9

   Plasmid

   #163526

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET1.2-U3

   Plasmid

   #173156

   Purpose

   Single guide RNA cassette under U3 promoter

   Depositor

   Piotr Ziolkowski

   Article

   Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

   Insert

   sgRNA cassette under U3 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   promoter region of the U3C snRNA gene (Marshallsa…

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127315

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

   Plasmid

   #127312

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    aac(3)IV (apramycin resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_APOBEC1-UdgX-nCas9

    Plasmid

    #163531

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLC-EGFP-RIP1

    Plasmid

    #75163

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIPk1

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIPK1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS::AtHAP2-V5

    Plasmid

    #132958

    Purpose

    Express AtHAP2 full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    AtHAP2 (GCS1, HAP2 Mustard Weed)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_EE-nCas9

    Plasmid

    #163541

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST_CAHS3_HYPDU (pBS0333)

    Plasmid

    #185179

    Purpose

    For the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS3_HYPDU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST_APOE_HUMAN_E114I (pBS0839)

    Plasmid

    #185274

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_E114I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E114I

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-miniSOG-VAMP2-citrine

    Plasmid

    #50969

    Purpose

    AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    miniSOG-VAMP2-citrine (Vamp2 Arabdopsis thaliana, Mouse)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST_AfrLEA3m (pBS0911)

    Plasmid

    #185285

    Purpose

    For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfrLEA3m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST_PvLEA4_repeats_k3_26 (pBS0762)

    Plasmid

    #185244

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid

    #141108

    Purpose

    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

    Depositor

    Matthew Gibson

    Article

    Hill et al Methods Mol Biol. 2022;2450:437-465. doi

    Insert

    Nematostella codon optimized Cas9
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    All amino acids coded for by codons optimized for…

    Promoter

    T7

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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