We narrowed to 23,164 results for: his

Showing: 3441 - 3480 of 23164 results

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YIp128-CUP1-His6-CUbo-GFP

Plasmid

#212792

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo-GFP

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-H2B-His8

Plasmid

#212810

Purpose

Constitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and NUbo

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo

Plasmid

#212812

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and CUbo

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-His6

Plasmid

#212819

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Tags

CUbo-6xHis

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo(K63R)-MCS

Plasmid

#212727

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo(K48R)-MCS

Plasmid

#212726

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K63R)-GFP

Plasmid

#212722

Purpose

Bacterial expression of His6-CUbo(K63R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K63R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K48R)-GFP

Plasmid

#212721

Purpose

Bacterial expression of His6-CUbo(K48R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K48R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET16b-RPA4/His14 (MSW#949)

Plasmid

#208075

Purpose

Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4, RPA14(His-tagged)

Tags

10xHis tag on RPA14

Expression

Bacterial

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

Plasmid

#201552

Purpose

Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1

Tags

HaloTag

Expression

Insect

Mutation

changed lysine 282 to arginine (numbering based o…

Promoter

Ac5

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Histone H3 pThr11 [N123/48R]

Plasmid

#206529

Purpose

Mammalian Expression Plasmid of anti-Histone H3 pThr11 (Rat). Derived from hybridoma N123/48.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H3 pThr11 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Hist1h2ail Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Histone H3 pThr11 [N123/19R]

Plasmid

#206528

Purpose

Mammalian Expression Plasmid of anti-Histone H3 pThr11 (Rat). Derived from hybridoma N123/19.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H3 pThr11 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Hist1h2ail Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab12 Q101L

Plasmid

#207347

Purpose

Bacterial expression of Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

Rab12 (RAB12 Human)

Tags

2xHis

Expression

Bacterial

Mutation

Q101L

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO Δ37 PPM1H

Plasmid

#207334

Purpose

Bacterial expression of His SUMO Δ37 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Δ37

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO Rab10(Q68L)

Plasmid

#207342

Purpose

Bacterial expression of His SUMO Rab10(Q68L)

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab10 (RAB10 Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Q68L

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaR-SpCas9-NLS-6xHis

Plasmid

#207388

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A, amino acids 10…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-SpCas9-HF1-NLS-6xHis

Plasmid

#207382

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-His-TEV-A7-Strep (2-180)

Plasmid

#190067

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Tags

GB1-His-TEV and Strep

Expression

Bacterial

Mutation

PRD only, residues 2-180

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDL28_Cas9-His Δcys E532C/E1207C

Plasmid

#206289

Purpose

Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E1207C

Promoter

T7

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDL27_Cas9-His Δcys E532C/E945C

Plasmid

#206288

Purpose

Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E945C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDL26_Cas9-His Δcys M1C/E532C

Plasmid

#206287

Purpose

Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

M1C, C80S, E532C, C574S

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His8-aCat(660-865)

Plasmid

#203335

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-865 only

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His8-aCat(660-885)

Plasmid

#203334

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-885 only

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCI_E01_mini-mAgrin-Myc-His-WPRE_v2

Plasmid

#198128

Purpose

Express mini-Agrin in mammalian cells

Depositor

Jeffrey Miner

Insert

mini-Agrin (Agrn Mouse)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa LCD

Plasmid

#197014

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET-20b_HtrA2(delta(133))_S306A_6His

Plasmid

#197975

Purpose

E. coli expression of HtrA2(delta133)-6His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

HtrA2 (HTRA2 Human)

Tags

6His

Expression

Bacterial

Mutation

delta(1-133), S306A

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI_E01_mini-mAgrin-Myc-His-WPRE__LG2

Plasmid

#198139

Purpose

Express mini-Agrin in mammalian cells

Depositor

Jeffrey Miner

Insert

mini-Agrin (Agrn Mouse)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI_E01_NtA-Fs1-Myc-His-WPRE

Plasmid

#198132

Purpose

Express NtA-Fs1 in mammalian cells

Depositor

Jeffrey Miner

Insert

NtA-Fs1

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1642-GB1His6_AH

Plasmid

#148890

Purpose

Bacterial Expression of HsHelz_1475-1642

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1642 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1942-GB1His6_AH

Plasmid

#148891

Purpose

Bacterial Expression of HsHelz_1475-1942

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1942 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsHelz_1643-1792-GB1His6_AH

Plasmid

#148893

Purpose

Bacterial Expression of HsHelz_1643-1792

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1643-1792 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsHelz_1793-1914-GB1His6_AH

Plasmid

#148894

Purpose

Bacterial Expression of HsHelz_1793-1914

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1914 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsHELZ_1793-1942-GB1His6_AH

Plasmid

#148896

Purpose

Bacterial Expression of HsHelz_1793-1942

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1942 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsHelz_1051-1474-GB1His6_AG

Plasmid

#148798

Purpose

Bacterial Expression of HsHelz_1051-1474

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1051-1474 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmRoquin_702-819-GB1His6_AE

Plasmid

#148624

Purpose

Bacterial Expression of DmRoquin_702-819

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-DmRoquin_501-702-GB1His6_AE

Plasmid

#148620

Purpose

Bacterial Expression of DmRoquin_501-702

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_501-702 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 23,164 results for: his