We narrowed to 2,365 results for: DAP

1. lentiCRISPRv2_sgCtrl#2

   Plasmid

   #174149

   Purpose

   Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Blast-3xHA-LMNB1

   Plasmid

   #207778

   Purpose

   Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. qTAG-N-Puro-mStayGold-ACTB

   Plasmid

   #227320

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-Solo-mStayGold-CLTC

   Plasmid

   #227313

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Blast-ultraID-LMNB1

   Plasmid

   #207775

   Purpose

   Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. qTAG-C-moxGFP-Puro-MAPRE1

   Plasmid

   #207794

   Purpose

   Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. qTAG-N-Puro-mScarlet-ACTB

   Plasmid

   #207754

   Purpose

   Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCMV5-FLAG-Gene33

   Plasmid

   #21633

   Depositor

   John Kyriakis

   Article

   Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

   Insert

   Gene33 (Errfi1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pKHH030_sgRB1#2

   Plasmid

   #174145

   Purpose

   Lentiviral vector expressing a sgRNA against the human RB1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   RB1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV-NSD3-short W284A

    Plasmid

    #72551

    Purpose

    expresses 3*FLAG tagged human NSD3-short with W284A mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    W284A

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. R-Q111R-N122D

    Plasmid

    #167178

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-R-Q111R-N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed R at 111 to Q and changed D at 122 to N

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR v2-Nr4a1sgRNA1

    Plasmid

    #160945

    Purpose

    Guide RNA 1 to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 gRNA 1 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. integrin alpha9 / alpha5 pcDNA1 neo

    Plasmid

    #13588

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV-NSD3-short del(1-100)

    Plasmid

    #72547

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    1-100 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

    Plasmid

    #128278

    Purpose

    Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

    Depositor

    Anna Sokac

    Article

    Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

    Insert

    twinstar (tsr) (tsr Fly)
    

    Use

    Tags

    6X Histidine tag

    Expression

    Bacterial

    Mutation

    Met1 changed to Pro1; Cys77 silent basepair mutat…

    Promoter

    T7

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. integrin alpha9 / alpha4 pcDNA1 neo

    Plasmid

    #13587

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 4 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. integrin alpha9 / alpha2 pcDNA1 neo

    Plasmid

    #13586

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 2 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Str-KDEL_SBP-EGFP-LAMP1-Y414A

    Plasmid

    #222319

    Purpose

    Synchronize the trafficking of LAMP1-Y414A from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tyr414 to Ala

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Str-KDEL_SBP-mCherry-LAMP1-Y414A

    Plasmid

    #222320

    Purpose

    Synchronize the trafficking of LAMP1-A17 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-N-Solo-mStayGold-LMNB1

    Plasmid

    #227328

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge