We narrowed to 1,992 results for: rp1

1. pLJC2-DHFR-3xFLAG

   Plasmid

   #217428

   Purpose

   Lentiviral overexpression of human DHFR

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   DHFR (DHFR Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Insert sequence is a codon-optimized gBLOCK (IDT)

   Promoter

   CMV

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

   Plasmid

   #225222

   Purpose

   Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   α-Synuclein
   Aga2
   

   Use

   Tags

   GS linker, HA Tag, and c-myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRP.ABCA4 c.5461-10T

   Plasmid

   #226553

   Purpose

   Minigene encoding the ABCA4 c.5461-10T mutation

   Depositor

   Dave Wee

   Article

   Tommaso et al Molecular Therapy: Nucleic Acid (2025)

   Insert

   ABCA4 (ABCA4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.5461-10T intronic mutation

   Promoter

   CAG

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ A-MFN2-Strep (SB253)

   Plasmid

   #227602

   Purpose

   Inducible expression of Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   Twin-StrepTag, 10xHis

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAPM-miR30-LRPPRC-ts3

   Plasmid

   #174270

   Purpose

   LRPPRC knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   LRPPRC shRNA (LRPPRC Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1928

   Plasmid

   #142291

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   XRCC5 (XRCC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF3469

   Plasmid

   #144945

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF438 (ZNF438 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2104

   Plasmid

   #143569

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB34 (ZBTB34 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2017

   Plasmid

   #142310

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PITX2 (PITX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBT9-ATG9A-N

    Plasmid

    #198530

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBT9-FTS

    Plasmid

    #198526

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FTS (AKTIP Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBT9-FHIP-L

    Plasmid

    #198528

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FHIP-L (FHIP1A Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBT9-ubiquitin

    Plasmid

    #196999

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    ubiquitin (UBC Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0923

    Plasmid

    #141756

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2F2 (NR2F2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid

    #184636

    Purpose

    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Gat1 gene with Arginine 69 changed to Lysine, to …

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

    Plasmid

    #166855

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K683E, H685E, K686E

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

    Plasmid

    #166854

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    F641E, I645E

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

    Plasmid

    #166853

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    F641G, I645G

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAL4-DBD-HEY2wt-IDR

    Plasmid

    #145235

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HEY2wt-IDR (HEY2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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