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Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
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pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneUseTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LT1_33_pTEFmut8-mCherry
Plasmid#177706PurposeConstruct for genome integration expressing constitutively mCherry under pTEFmut8 promoterDepositorInsertpTEFmut8-mCherry
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1
Plasmid#41595DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSII42K
Plasmid#194523PurposeHigh copy vector backbone with G418 selectivity, contains MCSDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIIIA/MS2-1
Plasmid#220631PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIIIA/MS2-2
Plasmid#220632PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::URA3
Plasmid#227595PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::URA3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT2-HRP
Plasmid#44021DepositorInsertHRP
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03
Plasmid#28003DepositorInsertAT1.03
UseTagsmVenus and mseCFPExpressionYeastMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPICZalphaB-SapL3
Plasmid#78171PurposeExpression of Saporin L3 in pichiaDepositorInsertSaporin L3
UseTagsalpha factorExpressionYeastMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a LEU2
Plasmid#61923PurposeExpression of K. lactis LEU2 cloned into pFA6aDepositorInsertLEU2
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a URA3
Plasmid#61924PurposeExpression of S. cerevisiae URA3 cloned into pFA6aDepositorInsertURA3
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS405-LEU2-TEF-KpolDcr1v2
Plasmid#85193PurposeIntegrating plasmid, K. polysporus Dcr1 under TEF promoterDepositorInsertDcr1
UseIntegrating, shuttle vectorTagsExpressionYeastMutationPromoterTEFAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPH1-mEGFP
Plasmid#105270PurposePlasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.DepositorInsertVph1 portion (VPH1 Budding Yeast)
UseTagsmEGFPExpressionYeastMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLII12Pglu-700μδ6
Plasmid#28002DepositorInsertFLII12Pglu-700μδ6
UseTagseCFP and eYFPExpressionYeastMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS426GFP-2×PH(PLCδ)
Plasmid#36092DepositorInsert2xPH(PLCdelta) fusion
UseTagsGFPExpressionYeastMutationcontains aa11-140 of PLCδ (including the PH domai…PromoterPRC1Available sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB8644
Plasmid#126919PurposePlasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYBDepositorInsertXdCrtI and HMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8787
Plasmid#126921PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1DepositorInsertHMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK113
Plasmid#200144PurposeType 4a part for Modular Cloning in YeastDepositorInsertsfGFP1-10
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEDJ333
Plasmid#177272PurposeGAL1p:Cas9:CYC1tDepositorInsertCas9
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB8748
Plasmid#126918PurposePlasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtEDepositorInsertXdCrtE
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8788
Plasmid#126922PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1DepositorInsertXdCrtI and HMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8786
Plasmid#126920PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtIDepositorInsertXdCrtI
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIII/MS2-2
Plasmid#220630PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS2-1
Plasmid#220627PurposePlasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only