We narrowed to 5,033 results for: plasmid dna
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Plasmid#69705PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ-BR11 dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-U3
Plasmid#69701PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA RBS+ emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-U4
Plasmid#69702PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi RBS+ CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-V
Plasmid#69703PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ-BR13 dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-V2
Plasmid#69704PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ-BRM1 dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-U2
Plasmid#69700PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 RBS+ dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B DN
Plasmid#52243PurposeDrosophila expression of YFP-Ras64B dominant negative mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS19N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B CA
Plasmid#52244PurposeDrosophila expression of YFP-Ras64B constitutively active mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ63L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-V5His6_D
Plasmid#146095PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionInsectMutationseven silent mutations compared to the sequence g…PromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
RF-227-UB-BMX
Plasmid#79186PurposeExpression of the C-terminal fragment of BMX that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBMX (BMX Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-242PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
MP-P11
Plasmid#69670PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 mutSdN
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tau (P301L)-VC
Plasmid#87633Purposeexpresses hTau fused to the C-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationMutation P301LPromoterCMVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
VN-Tau (P301L)
Plasmid#87634Purposeexpresses hTau fused to the N-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationMutation P301LPromoterCMVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc
Plasmid#161585Purposeexpression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutationDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationG2019SPromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
hGli3 6x his
Plasmid#84919Purposemammalian expression of Gli3DepositorInsertGli3 (GLI3 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc
Plasmid#161584Purposeexpression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutationDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationD1994SPromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PtenK13E/K289E-Puro
Plasmid#135681PurposeConstitutive expression (cMV promoter) of K13E/K289E mutant Pten cDNA, Puromycin selectionDepositorInsertPten (Pten Mouse)
UseLentiviralTagsExpressionMutationK13E/K289EPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDZ2FE_Champ1_1-812
Plasmid#105675PurposeExpresses Flag-tagged full-length human Champ1 proteinDepositorInsertChamp1 (CHAMP1 Human)
UseTags2X Flag tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(NcoI/EcoRI)
Plasmid#15405DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationinsert can be released by digestion with REs NcoI…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc
Plasmid#161587Purposeexpression of Myc-tagged mouse Lrrk2 without GTPase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1023-1879PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
Plasmid#161586Purposeexpression of Myc-tagged mouse Lrrk2 without kinase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1871-2540PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPABPC1-V5His6_G
Plasmid#146368PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and seven silent mu…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPABPC1-V5His6_G
Plasmid#146352PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and seven silent mu…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCJH059_proD_Cas12c_DualRNA-GFP_CAM
Plasmid#183076PurposePlasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.DepositorInsertCas12c tracrRNA and pre-crRNA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH019_SC101_pLAC_Cas12c(4)_Kan
Plasmid#183071PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c_4
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH021_SC101_pLAC_Cas12c2_Kan
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-dsRNAres-V5His6_O
Plasmid#147069PurposeInsect Expression of DmPABPC1-dsRNAresDepositorInsertDmPABPC1-dsRNAres (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S and four silent muta…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H127
Plasmid#170343Purpose6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsert6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJH009_proD_Cas12c-sgRNA-GFP_CAM
Plasmid#183070PurposePlasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertCas12c single guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH031_proD_LbCas12a-gRNA-GFP_CAM
Plasmid#183075PurposePlasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertLbCas12a guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmFMR1_B
Plasmid#145920PurposeInsect Expression of DmFMR1DepositorInsertDmFMR1 (Fmr1 Fly)
UseTagsExpressionInsectMutationTwo amino acid mutations (T214I and N501S) and th…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmFMR1_B
Plasmid#145940PurposeInsect Expression of DmFMR1DepositorInsertDmFMR1 (Fmr1 Fly)
UseTagsExpressionInsectMutationTwo amino acid mutations (N45D and G558R) and thr…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDZ2FE_Champ1_1-812_2PA
Plasmid#105676PurposeExpresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 bindingDepositorInsertChamp1 (CHAMP1 Human)
UseTags2X Flag tagExpressionMammalianMutationMutations: P336A, P341APromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAK_HUMAN_D0
Plasmid#79682PurposeThis plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertGAK (GAK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH21-Tier1-PhEF1a
Plasmid#169561PurposeTier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA Display H3_A/Hong Kong/1/1968
Plasmid#206506PurposeExpresses H3_Hong Kong/1/1968 in the surface of mammalian cellsDepositorInsertH3_A/Hong Kong/1/1968
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationdeleted amino acids 1651 to 1735PromoterCMVAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2364-Tier1-IRES
Plasmid#169562PurposeTier-1 vector carrying an internal ribosome entry site (IRES-pA)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001146687)
Plasmid#76470Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorInsertGAK (GAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001146263)
Plasmid#76471Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorInsertGAK (GAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001148048)
Plasmid#76472Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorInsertGAK (GAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only