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We narrowed to 40,618 results for: TRO

Showing: 4351 - 4400 of 40618 results

  6. SG3ΔENV CA E45A

    Plasmid
    #145783
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).
    Depositor
    Wesley Sundquist
    Article
    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/
    Insert
    Capsid
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Capsid residue glutamic acid 45 to alanin…
    Promoter
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    145783-plasmid-map-sequence-id-313267

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  9. pcDNA3-ER-CaNAR2

    Plasmid
    #64732
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal 27 amino acids from CYP450,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64732-plasmid-map-sequence-id-109351

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  11. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    Lyn-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64730-plasmid-map-sequence-id-109348

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  12. AAV-tTA

    Plasmid
    #175280
    Purpose
    AAV vector mediating neuron-specific expression of tTA
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    tTA advanced (improved tetracycline-controlled transactivator)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175280-plasmid-map-sequence-id-349089

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  16. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Promoter
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    40306-plasmid-map-sequence-id-115990

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  17. VinTS-tCRMod-GGSGGS9

    Plasmid
    #111765
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module
    Depositor
    Brenton Hoffman
    Article
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111765-plasmid-map-sequence-id-214720

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  18. TRIN-shREN

    Plasmid
    #105586
    Purpose
    Dox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistance
    Depositor
    Christopher Vakoc
    Article
    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.
    Insert
    control shRNA targeting luciferase
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. SG3ΔENV RT D185A

    Plasmid
    #145782
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).
    Depositor
    Wesley Sundquist
    Article
    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/
    Insert
    Reverse Transcriptase
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Reverse Transcriptase Aspartic Acid 185 t…
    Promoter
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    145782-plasmid-map-sequence-id-352197

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  28. VinTS-tCRMod-GGSGGS7

    Plasmid
    #111764
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module
    Depositor
    Brenton Hoffman
    Article
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111764-plasmid-map-sequence-id-214718

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  32. pAD-SPS6-Pman

    Plasmid
    #133906
    Purpose
    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    sfGFP(SPS6)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133906-plasmid-map-sequence-id-261727

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  35. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  37. pAAV-BRX-eGFP-NLS

    Plasmid
    #217534
    Purpose
    AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    BMP reporter element and miniXon casette (Smad1 Mouse)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    BMP Reporter Element
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217534-plasmid-map-sequence-id-429604

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  43. pcDNA3-Mito-CaNAR2

    Plasmid
    #64731
    Purpose
    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CaNAR2
    Use
    Tags
    Cerulean3, N-terminal 30 amino acids from DAKAP1,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64731-plasmid-map-sequence-id-111768

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  44. pU6T7

    Plasmid
    #71462
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)
    Depositor
    Willie Mark
    Article
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    human U6/phage T7
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    71462-plasmid-map-sequence-id-128762

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  45. pDN80

    Plasmid
    #61349
    Purpose
    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.
    Depositor
    Roland Eils, Barbara Di Ventura
    Article
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    AsLOV2 wt and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61349-plasmid-map-sequence-id-99714

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Showing: 4351 - 4400 of 40618 results