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pEGFP-C3_SEPT6

Plasmid

#71583

Purpose

mammalian expression of human SEPT6 with a GFP fusion

Depositor

Elias Spiliotis

Article

Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. d

Insert

Septin 6 (SEPTIN6 Human)

Tags

eGFP

Expression

Mammalian

Mutation

V206D

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT REV1

Plasmid

#201586

Purpose

Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-TsCC(B)::KANMX

Plasmid

#176397

Purpose

Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-TsCC(B)

Tags

mScarlet fused to TsCC(B)

Expression

Bacterial and Yeast

Promoter

none

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172503

Purpose

Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pscALPS- HIV2-ROD vpx

Plasmid

#115816

Purpose

Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV2ROD Vpx (M15390)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-mDNMT1_nick-lenti

Plasmid

#135959

Purpose

S. pyogenes nicking sgRNA for mouse DNMT1

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

mDNMT1_nick

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-HiBiT-N

Plasmid

#215815

Purpose

Expresses N protein with HiBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 HiBiT Nucleocapsid (N )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDF0352 HvsCas7-11 with C-term Nes mammalian expression

Plasmid

#172516

Purpose

Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

C-term Nes HvsCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TEV-GFP-FLAG LIC cloning vector (6LD)

Plasmid

#166835

Purpose

Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Type

Empty backbone

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag/HA-TRBP-L326A/Y358A

Plasmid

#215892

Purpose

Expression of TRBP-L326A/Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A/Y358A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPS-HIV-1 Group P-vpr

Plasmid

#115837

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group P Vpr (HQ179987)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0336 pAAV EFS-HvsCas7-11 for dual vector

Plasmid

#172515

Purpose

Encodes HvsCas7-11 mammalian expression casette in AAV backbone

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV EFS-HvsCas7-11 for dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172505

Purpose

Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-E

Plasmid

#215814

Purpose

Expresses SARS-CoV-2 Envelope protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Envelope (E )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-M

Plasmid

#215813

Purpose

Expresses SARS-CoV-2 Membrane protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Membrane (M )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS gag-gfp/vpx

Plasmid

#115808

Purpose

SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMT2-HA-MKK3

Plasmid

#21580

Depositor

John Kyriakis

Article

Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-

Insert

mitogen-activated protein kinase kinase 3 (MAP2K3 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pscALPS-HIV-1 Group O-vpr

Plasmid

#115836

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group O Vpr (L20571)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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