We narrowed to 6,931 results for: crispr cas9 plasmids

1. pLenti-sgRNA-Lib

   Plasmid

   #53121

   Purpose

   sgRNA expression construction in a 3rd generation lentiviral backbone

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORFE1001

   Plasmid

   #112079

   Purpose

   Overexpression of the AtCAS9 in plant under 2x35S promoter

   Depositor

   Christian Chevalier

   Insert

   AtCAS9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Plant codon optimized Cas9 S. pyogenes

   Promoter

   2x35S

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSc1-puro

   Plasmid

   #80438

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6, and hPGK

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin and U6

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-BbsI-chiRNA

   Plasmid

   #45946

   Purpose

   Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

   Depositor

   Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   U6-BbsI-chiRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Dm-snRNA:U6:96Ab

   Available since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP2443

   Plasmid

   #72252

   Purpose

   Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_ZNF3_1

   Plasmid

   #72363

   Purpose

   Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   ZNF3 (ZNF3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458_ZNF3_2

   Plasmid

   #72364

   Purpose

   Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   ZNF3 (ZNF3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

    Plasmid

    #99679

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65(101-261)-RTA(125-190)

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS287

    Plasmid

    #73730

    Purpose

    SapTrap flexible linker C-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Flexible Linker
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMLS288

    Plasmid

    #73735

    Purpose

    SapTrap flexible linker N-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Flexible Linker
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMLS279

    Plasmid

    #73729

    Purpose

    SapTrap FLP-on C-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    C-terminal FLP-on
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMLS382

    Plasmid

    #73731

    Purpose

    SapTrap Flagtag-TEV C-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    FLAGtag-TEV
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMLS282

    Plasmid

    #73733

    Purpose

    SapTrap FLP-on N-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    N-terminal FLP-on
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLS383

    Plasmid

    #73736

    Purpose

    SapTrap TEV-Flagtag N-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    TEV-FLAGtag
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-CASANOVA

    Plasmid

    #113036

    Purpose

    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDonor_sU6

    Plasmid

    #69351

    Purpose

    sgRNA scaffold and synthetic U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    sU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    synthetic U6 promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6>sgRNA(F+E)

    Plasmid

    #59986

    Purpose

    Empty vector for sgRNA cloning (F+E modified backbone)

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonor_mU6

    Plasmid

    #69350

    Purpose

    sgRNA scaffold and mouse U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    mU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mU6 promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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