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We narrowed to 10,632 results for: t7

Showing: 1741 - 1760 of 10632 results

  1. pSUMO-HsSyx3

    Plasmid
    #179288
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179288-plasmid-map-sequence-id-354924

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  2. pGEMHE-MmCENPT-4NS-GFP

    Plasmid
    #237431
    Purpose
    Expresses Mus musculus CENP-T with 4 rat-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with 4 rat-specific residues in HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    432 T to A; 463 I to T, 470 L to E, 485 Q to L
    Promoter
    T7
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    237431-plasmid-map-sequence-id-474223

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  3. pGEMHE-MmCENPT-RnHFD-4NS-GFP

    Plasmid
    #237432
    Purpose
    Expresses Mus musculus CENP-T with rat HFD and 4 mouse-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rattus norvegicus HFD and 4 mouse-specific residues in HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    432 A to T; 463 T to I, 470 E to L, 485 L to Q in…
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237432-plasmid-map-sequence-id-474252

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  4. E. coli Lig-N-HiBiT

    Plasmid
    #215437
    Purpose
    Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215437-plasmid-map-sequence-id-428719

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  5. E. coli Lig-C-HiBiT

    Plasmid
    #215438
    Purpose
    Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215438-plasmid-map-sequence-id-428720

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  6. pGEMHE-RnCENP-O-80-GFP

    Plasmid
    #205182
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Michael Lampson
    Article
    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208
    Insert
    CENP-O (Cenpo, Cenpo Mouse, Rat)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O N terminal …
    Promoter
    T7
    Available Since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205182-plasmid-map-sequence-id-409734

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  7. pGFP-HsSyx3

    Plasmid
    #179289
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + GFP + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    179289-plasmid-map-sequence-id-354922

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  8. pHsSyx3-MBP

    Plasmid
    #179286
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    TEV protease cleavage site + mature maltose bindi…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179286-plasmid-map-sequence-id-354920

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  12. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid
    #218190
    Purpose
    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Insert
    nucA
    Tags
    His and NSP4 signal secretion peptide
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218190-plasmid-map-sequence-id-467313

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  15. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  17. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  18. pTXB3-Flag PLK1 T210D

    Plasmid
    #68272
    Purpose
    bacterial expression of Plk1 T210D mutant
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    T210D
    Promoter
    T7
    Available Since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. Pet28a_Synthetic_Human_H2A.1

    Plasmid
    #42634
    Depositor
    Joe Landry
    Insert
    Human H2A.1 (H2AC13 Human, Synthetic)
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42634-plasmid-map-sequence-id-57749

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  20. pAIP4_3KB5

    Plasmid
    #234225
    Purpose
    Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3KB5 (TRIM72 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234225-plasmid-map-sequence-id-464190

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Showing: 1741 - 1760 of 10632 results