We narrowed to 2,087 results for: Endog

Showing: 1761 - 1780 of 2087 results

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pNRCreBle

Plasmid

#234431

Purpose

Picochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal

Depositor

Matthew Posewitz

Article

Burch et al Front Microbiol. 2025 Jul 14;16:1588625.

Insert

Cre recombinase

Use

CRISPR, Cre/Lox, and Synthetic Biology ; Green al…

Mutation

Picochlorum celeri endoglucanase intron insertion

Promoter

P celeri nitrate reductase

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-HIS3

Plasmid

#229230

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-NatMX6

Plasmid

#229231

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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N-Terminal Halo XRCC4

Plasmid

#207541

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-Ku70

Plasmid

#207547

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with flanked by human Ku70 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago2

Plasmid

#170918

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago1

Plasmid

#170919

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585_Y167F

Plasmid

#174402

Purpose

Bacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Mutation

sarA with Y167F mutation

Promoter

Endogenous

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 Δ5H

Plasmid

#163582

Purpose

express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

L255A; mutate 440-450, 489-500, 701-708, 827-831,…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H

Plasmid

#163581

Purpose

express Dcp2C Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-300; mutate aa440-450, aa489-50…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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EZH2-Promoter-gRNA

Plasmid

#131329

Purpose

gRNA to generate endogenously tagged -tetR-EZH2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EZH2-Promoter-gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTJK502

Plasmid

#121457

Purpose

CEN HIS3 CNB1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Promoter

Endogenous genomic promoter region

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK513

Plasmid

#121470

Purpose

CEN LEU2 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK511

Plasmid

#121468

Purpose

2µm HIS3 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK510

Plasmid

#121465

Purpose

2µm HIS3 CNB1mutEF3

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF3 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 103 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK509

Plasmid

#121464

Purpose

2µm HIS3 CNB1mutEF2

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 66 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK508

Plasmid

#121463

Purpose

2µm HIS3 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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