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pGK:TRL-EFS:iRFP670

Plasmid

#68475

Purpose

Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

tTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EFS and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Use

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pSI-359

Plasmid

#131131

Purpose

gRNA expression vector for A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

A-to-G reporter activation gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-miR-34 MT

Plasmid

#78259

Purpose

psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

Depositor

Joanne Weidhaas

Article

Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

Insert

mutated miR-34 target site

Use

Luciferase; Microrna activity

Tags

Expression

Mammalian

Mutation

nucleotides 2-5 and 10-11 changed from CCGT to GG…

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRZ-mNanog-YNL

Plasmid

#65717

Purpose

mNanog reporter plasmid (expresses YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Nanog promoter and YNL

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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piGECI-NES-N1

Plasmid

#160422

Purpose

Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDendra2-Hygro

Plasmid

#202407

Purpose

Dendra2 expression plasmid

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

Hygromycin

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPBpuro-EGFP-eDHFR(69K6)

Plasmid

#178850

Purpose

Mammalian expression of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT1

Plasmid

#106304

Purpose

Express SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 1 (SHMT1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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1200 pAdTrack FKHR H215R

Plasmid

#9038

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

H215R

Promoter

Available since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pZ005 (AacC2c1 human codon optimized protein)

Plasmid

#70171

Purpose

Expresses AacC2c1 human codon optimized protein

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 protein

Use

Tags

NLS

Expression

Mutation

Promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pMRXIP-Myc-SNAP29

Plasmid

#116944

Purpose

Expressing Myc-SNAP29 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-B-GECO1

Plasmid

#32469

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-CheRiff-T2A-mCherry

Plasmid

#170275

Purpose

CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

CheRiff-T2A-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

11xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Cyan Nano-lantern/pRSETB

Plasmid

#67658

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71344

Purpose

Express Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern(Ca2+)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only