We narrowed to 2,414 results for: organization Addgene

1. PhyB-mCherry-Spc72

   Plasmid

   #51582

   Purpose

   Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   Spc72 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

   Plasmid

   #127365

   Purpose

   Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   R118G

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

   Plasmid

   #127787

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32, Arg36 and Arg69 to Ala

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

   Plasmid

   #127363

   Purpose

   Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Plant

   Mutation

   R118G

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

   Plasmid

   #233546

   Purpose

   A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF1 (FOXF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

   Plasmid

   #232132

   Purpose

   A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF1 (FOXF1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IPU-Venus-GS27

   Plasmid

   #228138

   Purpose

   Expresses Venus-GS27 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   GS27 (GOSR2 Human)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IPU-Venus-Rab11

   Plasmid

   #228140

   Purpose

   Expresses Venus-Rab11 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   Rab11 (RAB11A Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBa.HALO-KIF3A tail 419-702

   Plasmid

   #193924

   Purpose

   Mammalian expression of Halo-tagged Kif 3A tail 419-702

   Depositor

   Marvin Bentley

   Article

   Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

   Insert

   Kif 3A (KIF3A Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBa.Halo-KIF3B tail 391-747

    Plasmid

    #193929

    Purpose

    Mammalian expression of Halo-tagged Kif 3B tail 391-747

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3B (Kif3b Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBa.GFP-KIF3A tail 419-702

    Plasmid

    #193923

    Purpose

    Mammalian expression of GFP-tagged Kif 3A tail 419-702

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3A (KIF3A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMH-HT_CtCHAD-H253A-R256A-R260A

    Plasmid

    #127788

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

    Plasmid

    #127789

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

    Plasmid

    #127792

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg3…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FAB(mito-LD)

    Plasmid

    #214425

    Purpose

    Detection of mito-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-3xHA-V5-mito
    

    Use

    Tags

    CFAST-Halo and RspAN-3XHA-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET_MBP-RGG-GFP-RGG

    Plasmid

    #124949

    Purpose

    Expression of MBP-RGG-GFP-RGG

    Depositor

    Daniel Hammer, Benjamin Schuster, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    EGFP, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-FlicR1

    Plasmid

    #74142

    Purpose

    Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-NPC1(WT)-FLAG

    Plasmid

    #164972

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-FAB(ER-mito)

    Plasmid

    #214423

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
    

    Use

    Lentiviral

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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