We narrowed to 7,531 results for: ski

1. pAPM-D4 miR30-AGO1 ts2

   Plasmid

   #115851

   Purpose

   AGO1 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   AGO1 shRNA (AGO1 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX-FHH-NRASQ61K/T35A-IRES-Puro

   Plasmid

   #120571

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K, T35A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

   Plasmid

   #120573

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K, Y40A

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX-FHH-NRASQ61K/E37A-IRES-Puro

   Plasmid

   #120572

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K, E37A

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAPM-D4 miR30-AGO2 ts3

   Plasmid

   #115855

   Purpose

   AGO2 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   AGO2 shRNA (AGO2 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3

   Plasmid

   #112843

   Purpose

   transient/stable overexpression of the C-terminal domain of Catulin.

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Catulin-alpha-1 (Ctnnal1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C2 domain of Catulin

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1

   Plasmid

   #112841

   Purpose

   transient/stable overexpression of the N-terminal domain of Catulin.

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Catulin-alpha-1 (Ctnnal1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N domain of Catulin

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

   Plasmid

   #229498

   Purpose

   This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   MYC 5'Untranslated region + destabilized GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-syn-FLEX-jYCaMP1s

   Plasmid

   #135420

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

    Plasmid

    #89991

    Purpose

    Donor template for generation of SOX2-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

    Plasmid

    #120565

    Purpose

    Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

    Plasmid

    #120567

    Purpose

    Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, Y40A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

    Plasmid

    #120566

    Purpose

    Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, E37A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET iGluSnFR3 v857

    Plasmid

    #175186

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v857
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-hACE2-hygro

    Plasmid

    #161758

    Purpose

    Expresses human ACE2 in mammalian cells

    Depositor

    Neville Sanjana

    Article

    Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pJEP313-pAAV-CMV-SaCas9-DIO-pA

    Plasmid

    #113690

    Purpose

    A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET iGluSnFR3 v82

    Plasmid

    #175187

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v82
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82706

    Purpose

    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    U6/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-Blast POLE1

    Plasmid

    #160802

    Purpose

    Express POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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