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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

Plasmid

#78353

Purpose

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Human, Synthetic)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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ATM gRNA (BRDN0001146099)

Plasmid

#77530

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ATM (ATM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ATM gRNA (BRDN0001149033)

Plasmid

#77531

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ATM (ATM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT1 gRNA (BRDN0001146935)

Plasmid

#75500

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT1 (AKT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

Plasmid

#78509

Purpose

Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD45R0Ex (PTPRC Human)

Tags

His10 and SNAPf

Expression

Insect

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform5

Plasmid

#70177

Purpose

Mammalian expression of hsOPA1 isoform 5

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 5 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GNPTAB

Plasmid

#78108

Purpose

Mammalian expression of human GNPTAB with C terminal myc tag

Depositor

Thomas Braulke

Article

Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

Insert

GNPTAB (GNPTAB Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-149

Plasmid

#73410

Purpose

Expresses HA tagged aa1-149 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-149 only of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only