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178,051 results

Showing: 8941 - 8950 of 178051 results

  4. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  6. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid
    #174649
    Purpose
    Live-cell marker for stable microtubules
    Depositor
    Lukas Kapitein
    Publication
    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    Tags
    mNeongreen-mNeongreen
    Expression
    Mammalian
    Mutation
    rigor mutation G234A
    Promoter
    Chicken beta-actin
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174649-plasmid-map-sequence-id-345295

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  7. pDAC446

    Plasmid
    #195239
    Purpose
    Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Publication
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Expression
    Mammalian
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195239-plasmid-map-sequence-id-382947

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  9. mito-meGFP

    Plasmid
    #172481
    Purpose
    meGFP targeted to the mitochondrial matrix with a human COX8 presequence
    Depositor
    Thomas Schwarz
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mito (COX8A Human)
    Tags
    meGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172481-plasmid-map-sequence-id-345344

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  10. G13-CASE

    Plasmid
    #168127
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F125/D126 within GNA13
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168127-plasmid-map-sequence-id-332872

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Showing: 8941 - 8950 of 178051 results