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pcDNA3+ hSlo2.2
Plasmid#16214DepositorInsertKCNT1 potassium channel, subfamily (KCNT1 Human)
UseTagsExpressionMammalianMutationThis construct lacks the first 53aa when compared…PromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pOX + hSlo2.2
Plasmid#16215DepositorInsertKCNT1 potassium channel, subfamily (KCNT1 Human)
UseXenopus oocyte expressionTagsExpressionMutationThis construct lacks the first 53aa when compared…PromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
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JMC01B-H12
Plasmid#58758Purposebacterial expression of PDB 4O62DepositorInsertPDB:4O62 (ZCWPW2 Human)
UseTagsHisExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceAug. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pKT2/CAGXSP/CD63mScarlet
Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorInsertCD63-mScarlet (CD63 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Puro-HEK3 CTT ins
Plasmid#171996PurposeDelivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
UseTagsExpressionMammalianMutationPromoterCMV for Cas9, U6 for gRNAsAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-LacZ-GFP-Puro (BB)
Plasmid#170459PurposeFor cloning gRNA blockDepositorInserthU6-BB-LacZ and EF1A-EGFP-2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 for gRNA and EF1A for EGFP-2A-PuroAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only