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Showing: 321 - 360 of 473 results

  1. pUC57-mini v2-HF24_F16

    Plasmid
    #195730
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#16 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195730-plasmid-map-sequence-id-385583

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  2. pUC57-mini v2-HF24_F18

    Plasmid
    #195732
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#18 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195732-plasmid-map-sequence-id-385582

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  3. pUC57-mini v2-HF24_F1

    Plasmid
    #195715
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#1 of a 24 fragment split in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195715-plasmid-map-sequence-id-385580

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  4. pUC57-mini v2-HF24_F9

    Plasmid
    #195723
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#9 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195723-plasmid-map-sequence-id-385578

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  5. pUC57-mini v2-HF24_F23

    Plasmid
    #195737
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#23 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195737-plasmid-map-sequence-id-385577

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  6. pUC57-mini v2-HF24_F21

    Plasmid
    #195735
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#21 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195735-plasmid-map-sequence-id-385575

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  7. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

    Plasmid
    #69100
    Purpose
    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP
    Depositor
    David Botstein
    Inserts
    yZ3EV cassette
    Z3 promoter
    yEGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69100-plasmid-map-sequence-id-122356

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  8. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    mTQ2=mturquoise2 blue fluorescent gene
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mutation
    Promoter
    Villin
    Available since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    67278-plasmid-map-sequence-id-115887

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  9. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  27. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid
    #70085
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70085-plasmid-map-sequence-id-129388

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  32. pTFG002-ACE2(Mut)-HA

    Plasmid
    #191570
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human expression. Also contai…
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191570-plasmid-map-sequence-id-441411

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  33. pTFG001-ACE2(WT)-HA

    Plasmid
    #191569
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191569-plasmid-map-sequence-id-441362

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  34. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166133
    Purpose
    This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Raphael Gaudin
    Article
    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep
    Insert
    hU6-gRNA and hH1-gRNA targeting cldn1 gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166133-plasmid-map-sequence-id-333282

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  35. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166134
    Purpose
    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Raphael Gaudin
    Article
    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep
    Insert
    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166134-plasmid-map-sequence-id-333283

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  39. pExp-TFSumo

    Plasmid
    #112576
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-TFSumo and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    112576-plasmid-map-sequence-id-255698

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Showing: 321 - 360 of 473 results