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Showing: 1 - 20 of 475 results

  1. pExp-TF

    Plasmid
    #112575
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-TriggerFactor and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112575-plasmid-map-sequence-id-217211

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  7. TF-bio-His

    Plasmid
    #52184
    Purpose
    Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TF (TF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52184-plasmid-map-sequence-id-80548

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  10. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pGreenII Tfs-qqr::LUC

    Plasmid
    #44466
    Purpose
    Expresses a luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutation
    Depositor
    Roger Hellens, Ross Johnson
    Insert
    A luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutation
    Use
    Luciferase; Plant transformation
    Tags
    Expression
    Mutation
    A disruption was made between the first and secon…
    Promoter
    35S Promoter
    Available since
    June 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44466-plasmid-map-sequence-id-60745

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  19. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

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  20. pAAV-anti-GFP(LaG17) PAGER(TF)

    Plasmid
    #229997
    Purpose
    Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229997-plasmid-map-sequence-id-457203

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Showing: 1 - 20 of 475 results