We narrowed to 14,583 results for: pET

1. pETM33_Nsp3c_SUD-M

   Plasmid

   #156470

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28CLY8

   Plasmid

   #21767

   Depositor

   Maarten Merkx

   Article

   Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

   Insert

   ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pET15b-YC

   Plasmid

   #107072

   Purpose

   Host vector to construct a YPer or CyPet fusion protein

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

   Insert

   YPet-CyPet fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET24a_UDP_Xase_E113

   Plasmid

   #73159

   Purpose

   Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

   Depositor

   Sandra Gabelli

   Article

   Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

   Insert

   pET24a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   change glutamate 113 to alanine

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pet23a_RBPMS_1_120

   Plasmid

   #65730

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 1-120

   Promoter

   T7

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a.MJ1594

   Plasmid

   #11413

   Depositor

   Sung-Hou Kim

   Article

   Wang et al Structure. 2001 Jan 10. 9(1):65-71.

   Insert

   phosphoserine phosphatase (MJ_1594 M. jannaschii)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pet23a_RBPMS_20_101

   Plasmid

   #65733

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 20-101

   Promoter

   T7

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETMutSdelta768

   Plasmid

   #13041

   Depositor

   Peggy Hsieh

   Article

   Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

   Insert

   MutS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted C-terminal aa residues 769 - 811

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET21a.MJ0697

   Plasmid

   #11411

   Depositor

   Sung-Hou Kim

   Article

   Wang et al EMBO J. 2000 Feb 1. 19(3):317-23.

   Insert

   fibrillarin homolog (MJ_0697 M. jannaschii)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M222T

   Promoter

   T7

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a.MJ1247

    Plasmid

    #11414

    Depositor

    Sung-Hou Kim

    Article

    Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

    Insert

    3-hexulose-6-phosphate isomerase (MJ_1247 M. jannaschii)
    

    Use

    Tags

    T7-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a.MJ0882

    Plasmid

    #11417

    Depositor

    Sung-Hou Kim

    Article

    Huang et al J Struct Funct Genomics. 2002 . 2(3):12

    Insert

    methyltransferase (MJ_0882 M. jannaschii)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CtkA_full_pET21a

    Plasmid

    #27428

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

13. CtkA_300_pET21a

    Plasmid

    #27429

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    residues 301-325 are truncated

    Promoter

    Available since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

14. CtkA_D155Q_pET21a

    Plasmid

    #27430

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D155Q

    Promoter

    Available since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

15. CtkA_D179Q_pET21a

    Plasmid

    #27431

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D179Q

    Promoter

    Available since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pET21a.MJ0307

    Plasmid

    #11415

    Depositor

    Sung-Hou Kim

    Article

    Cave et al Protein Sci. 2001 Feb . 10(2):384-96.

    Insert

    disulfide oxidoreductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pET21a.TM1363

    Plasmid

    #11420

    Depositor

    Sung-Hou Kim

    Article

    Shin et al J Mol Biol. 2004 Jul 30. 341(1):227-39.

    Insert

    peptide release factor I (prfA Thermotoga maritima)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pET22b_msfGFP_stag_KS

    Plasmid

    #237630

    Purpose

    For expression of msfGFP_stag_KS in Ecoli

    Depositor

    Denes Hnisz

    Insert

    stag_KS
    

    Use

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET22b_msfGFP_stag

    Plasmid

    #237629

    Purpose

    For expression of msfGFP_stag in Ecoli

    Depositor

    Denes Hnisz

    Insert

    stag
    

    Use

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_HCK

    Plasmid

    #224239

    Purpose

    Expression of human HCK Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    hck (HCK Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28b-M2

    Plasmid

    #225735

    Purpose

    To express M2 (219-399) in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 219-399

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET303_sumocodo_GFP_codoopti

    Plasmid

    #210393

    Purpose

    Express SUMO-GFP in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Tags

    SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pET28b-M1

    Plasmid

    #225734

    Purpose

    To express M1 (13-193) in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 13-193

    Promoter

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET27b+R

    Plasmid

    #188245

    Purpose

    Empty backbone vector for protein expression in Escherichia coli and his-tag purification

    Depositor

    Yiping Qi

    Article

    Fang et al Methods Mol Biol. 2023;2653:153-171. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET24a_SMOX

    Plasmid

    #186345

    Purpose

    C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

    Insert

    Spermine oxidase (SMOX Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28_LmFPPS

    Plasmid

    #186322

    Purpose

    pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    FPPS
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET50delCT_Vipp1_dL10Ala

    Plasmid

    #223817

    Purpose

    Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

    Depositor

    Carsten Sachse

    Insert

    Vipp1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    replacement of 10 aa in Hinge 2 with Ala

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a:ChrH

    Plasmid

    #225084

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Use

    Tags

    His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a:ChrI

    Plasmid

    #225085

    Purpose

    Expresses anthrone-oxygenase like enzyme ChrI in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrI
    

    Use

    Tags

    His6 and His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 300_pETcon_SARS2_FLip

    Plasmid

    #222230

    Purpose

    yeast surface display of the SARS-CoV-2 FLip variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 299_pETcon_SARS2_EG-5

    Plasmid

    #222232

    Purpose

    yeast surface display of the SARS-CoV-2 EG.5 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28Man

    Plasmid

    #203399

    Purpose

    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

    Depositor

    David Vocadlo

    Article

    Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

    Insert

    man2A
    

    Use

    Tags

    H6

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pETURALEU

    Plasmid

    #223207

    Purpose

    Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

    Depositor

    Sahand Jamal Rahi

    Article

    Stojkovic et al G3 (Bethesda). 2024 Dec 17:jkae297. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a_PA1472_Cterm_His

    Plasmid

    #220485

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a_speG_Nterm_His

    Plasmid

    #220478

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a_PA4114_Nterm_His

    Plasmid

    #220479

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a_PA1472_Nterm_His

    Plasmid

    #220480

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a_PA1377_Nterm_His

    Plasmid

    #220481

    Purpose

    heterologous expression of PA1377 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1377
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. OoH_pET29b

    Plasmid

    #215424

    Purpose

    Expression construct for OoH gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    OoH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Ph-NylC_pET28a

    Plasmid

    #215425

    Purpose

    Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Ph-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge