We narrowed to 176 results for: Araf

1. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE

   Plasmid

   #198511

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV_hSyn-PdCO-EGFP-WPRE

   Plasmid

   #198513

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV_hSyn-PdCO-mScarlet-WPRE

   Plasmid

   #198510

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

   Plasmid

   #198515

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGGG-Sr43

   Plasmid

   #186974

   Purpose

   Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

   Depositor

   Brande Wulff

   Article

   Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

   Insert

   Sr43
   

   Use

   Tags

   None

   Expression

   Bacterial and Plant

   Mutation

   None

   Promoter

   Natice

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

   Plasmid

   #198508

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIα minimal promotor (0.4kb)

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

   Plasmid

   #198514

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIα minimal promotor (0.4kb)

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-GSDMD_1-270

   Plasmid

   #218229

   Purpose

   Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 1-270

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pcDNA3.1-GSDMD_1-275

   Plasmid

   #218230

   Purpose

   Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 1-275

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA3.1-GSDMD_29-484

    Plasmid

    #218231

    Purpose

    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-484

    Promoter

    Available since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pcDNA3.1-GSDMD_29-270

    Plasmid

    #218232

    Purpose

    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-270

    Promoter

    Available since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGGG_Sr1644-1Sh

    Plasmid

    #164087

    Purpose

    Expresses pGGG_Sr1644-1Sh in plants

    Depositor

    Brande Wulff

    Article

    Yu et al Nat Commun. 2022 Mar 25;13(1):1607. doi:

    Insert

    pGGG_Sr-1644-1Sh
    

    Use

    Tags

    none

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Native promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid

    #202199

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid

    #202198

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid

    #224697

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid

    #224698

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge