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Showing: 161 - 180 of 350 results

  1. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  3. pHD-DsRed-attP-w+

    Plasmid
    #80898
    Purpose
    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    80898-plasmid-map-sequence-id-172486

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  4. Mouse 5' Npm1 AID GFP PuroR

    Plasmid
    #127899
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 Gene
    Depositor
    Mark Groudine
    Insert
    Npm1 (Npm1 Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127899-plasmid-map-sequence-id-250993

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  5. AAV-KrasG12D/sgKras/Cre

    Plasmid
    #99851
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99851-plasmid-map-sequence-id-193760

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  7. AAV-KrasG12C/sgKras/Cre

    Plasmid
    #99850
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99850-plasmid-map-sequence-id-193772

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  8. AAV-KrasG12V/sgKras/Cre

    Plasmid
    #99854
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99854-plasmid-map-sequence-id-193790

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  9. AAV-KrasG13C/sgKras/Cre

    Plasmid
    #99856
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99856-plasmid-map-sequence-id-193794

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  10. AAV-KrasG12R/sgKras/Cre

    Plasmid
    #99852
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99852-plasmid-map-sequence-id-193774

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  11. AAV-KrasG13D/sgKras/Cre

    Plasmid
    #99857
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99857-plasmid-map-sequence-id-193798

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  12. AAV-KrasG12S/sgKras/Cre

    Plasmid
    #99853
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99853-plasmid-map-sequence-id-193755

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  13. AAV-KrasG12A/sgKras/Cre

    Plasmid
    #99849
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99849-plasmid-map-sequence-id-193789

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  15. AAV-KrasG13V/sgKras/Cre

    Plasmid
    #99860
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99860-plasmid-map-sequence-id-193770

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  16. AAV-KrasG13S/sgKras/Cre

    Plasmid
    #99859
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99859-plasmid-map-sequence-id-200267

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  17. AAV-KrasG13A/sgKras/Cre

    Plasmid
    #99855
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99855-plasmid-map-sequence-id-200262

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  18. AAV-KrasG13R/sgKras/Cre

    Plasmid
    #99858
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99858-plasmid-map-sequence-id-193748

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Showing: 161 - 180 of 350 results