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H11-mScarletALG8

Plasmid

#172440

Purpose

Hipp11 targeting vector, expresses mScarlet-mALG8

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mALG8

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDest17_hnRNPA1_YtoW

Plasmid

#224253

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_YtoW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_YtoW (HNRNPA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y52W, Y59W, Y75W, Y81W, Y110W, Y120W, Y129W

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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H11-mScarletLAMA4

Plasmid

#172439

Purpose

Hipp11 targeting vector, expresses mScarlet-mLAMA4

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mLAMA4

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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R26-dCas9-VPR-mNG

Plasmid

#172441

Purpose

Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

dCas9-VPR
mNeonGreen

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-VIM270-gSM

Plasmid

#215870

Purpose

This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

Depositor

Kumiko Ui-Tei

Article

Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

Insert

3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgfiller-eCas9-T2A-BlastR

Plasmid

#172437

Purpose

Expresses eCas9, BlastR and sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

eCas9
BlastR

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172434

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_hnRNPA1_FtoW

Plasmid

#224254

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_FtoW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_FtoW (HNRNPA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

F17W, F25W, F31W, F37W, F43W, F62W, F69W, F78W, F…

Promoter

T7

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-VIM270-pSM

Plasmid

#215871

Purpose

This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

Depositor

Kumiko Ui-Tei

Article

Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

Insert

3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172436

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
3X FLAG-FUS-WT

Plasmid

#44985

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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U6-sgH11-eCas9-T2A-BlastR

Plasmid

#172492

Purpose

For CRISPR-assisted HDR, Hipp11 locus

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

, BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-FUS-WT

Plasmid

#44978

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
U6-sgR26-eCas9-T2A-BlastR

Plasmid

#172493

Purpose

For CRISPR-assisted HDR, Rosa26 locus

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-Report-Luc-KLF4-K5S-Mt344A

Plasmid

#34601

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206B

Plasmid

#34600

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pDest17_hnRNPA1_allW

Plasmid

#224252

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_allW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_allW (HNRNPA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

F17W,F25W,F31W,F37W,F43W,Y52W, Y59W, F62W, F69W, …

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-Report-Luc-KLF4-K5S-Mt344B

Plasmid

#34602

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206A

Plasmid

#34599

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
GST-FUS 465-526

Plasmid

#44983

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (465-526aa) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 465-526

Promoter

tac

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only