We narrowed to 145,972 results for: ins;

1. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

   Plasmid

   #232343

   Purpose

   Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

   Depositor

   Philip Wong

   Article

   Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

   Insert

   HDGF like 2 (HDGFL2 Human)
   

   Use

   Tags

   GGGS linker, 6xHis and Twin-strep, TEV protease s…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEx-insertion-erm

   Plasmid

   #172210

   Purpose

   For genetic insertion in erythromycin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Genetic insertion in prevotella copri

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. INS-2A-luciferase-2A-tdT

   Plasmid

   #159348

   Purpose

   Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Insert

   Luciferase-2A-tdTomato
   

   Use

   Ins locus knockin donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

   Plasmid

   #197264

   Purpose

   Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTMEM-PhoCl-Insert

   Plasmid

   #219674

   Purpose

   Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

   Depositor

   Helge Ewers

   Article

   Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

   Insert

   TMEM-PhoCl-rtTA3
   

   Use

   Tags

   PhoCl

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1-ccdB-TwinStrep

   Plasmid

   #214988

   Purpose

   Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag

   Depositor

   Wade Harper

   Type

   Empty backbone

   Use

   Tags

   TwinStrep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-EGFR-Y764_V765insHH

   Plasmid

   #116319

   Purpose

   Lentiviral expression of EGFR Y764_V765insHH

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Y764_V765insHH

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRosa26-4xInsulator (JP6)

   Plasmid

   #99627

   Purpose

   IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCKan-INSyibp-Nef

   Plasmid

   #134593

   Purpose

   Low Phosphate inducible Nef

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   HIV Nef protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TwinStrep-SUMO-Pdp1

    Plasmid

    #198320

    Purpose

    For affinity purification of Pdp1 payload

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    TwinStrep-SUMO-Pdp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEx-insertion-tet

    Plasmid

    #172211

    Purpose

    For genetic insertion in tetracyclin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Genetic insertion in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBabe EGFR ASVinsertion

    Plasmid

    #32066

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert duplicate A767-V769

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBPK1520-HEK3-CTTins-ng

    Plasmid

    #180019

    Purpose

    Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U7

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TwinStrep-SUMO-Pnf

    Plasmid

    #198321

    Purpose

    For affinity purification of Pnf payload

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    TwinStrep-SUMO-Pnf
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pInsert-PhoCl-RER

    Plasmid

    #219676

    Purpose

    Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    CD4mScarlet-PhoCl-RER
    

    Use

    Tags

    mScarlet and PhoCl

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. ins-cre_cryaa-Venus

    Plasmid

    #24333

    Depositor

    Didier Stainier

    Article

    Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

    Insert

    Cre (cre )
    

    Use

    Cre/Lox

    Tags

    Venus

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-EGFR-E746_T751delinsA

    Plasmid

    #116246

    Purpose

    Lentiviral expression of EGFR E746_T751delinsA

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E746_T751delinsA

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-EGFR-E709_T710delinsD

    Plasmid

    #116239

    Purpose

    Lentiviral expression of EGFR E709_T710delinsD

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E709_T710delinsD

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 4xHRE_minSV40-sfGFP-CMV_dsRed

    Plasmid

    #117400

    Purpose

    sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe EGFR insertionH

    Plasmid

    #32067

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert H at H773-V774

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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