-
Plasmid#202476PurposeExpresses the nanobody E7 BoNT-A after phosphate depletionDepositorInsertnanobody E7 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-JM3
Plasmid#202477PurposeExpresses the nanobody JM3 after phosphate depletionDepositorInsertnanobody JM3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-J3
Plasmid#202478PurposeExpresses the nanobody J3 after phosphate depletionDepositorInsertnanobody J3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-m36.4
Plasmid#202479PurposeExpresses the nanobody m36.4 after phosphate depletionDepositorInsertnanobody m36.4
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re5D06
Plasmid#202480PurposeExpresses the nanobody Re5D06 after phosphate depletionDepositorInsertnanobody Re5D06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re9F06
Plasmid#202481PurposeExpresses the nanobody Re9F06 after phosphate depletionDepositorInsertnanobody Re9F06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-std
Plasmid#109417PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-mCherry
Plasmid#117752PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-G97 (for GFP)
Plasmid#130715PurposePlasmid encoding the bacterial expression of LAMA-G97 for GFPDepositorInsertLAMA-G97
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-N95 (for GFP)
Plasmid#130716PurposePlasmid encoding the bacterial expression of LAMA-N95 for GFPDepositorInsertLAMA-N95
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-aδNb072
Plasmid#204627PurposeExpresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.DepositorInsertaδNb072
UseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-2xTS
Plasmid#162779PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-myc
Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and mycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only