Addgene

Showing: 161 - 180 of 1457 results

Results per page:

Sort results by:

pHAGE-PIK3CA-P539R

Plasmid

#116525

Purpose

Lentiviral expression of PIK3CA P539R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

P539R

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-p53 (1-73) (72P)

Plasmid

#62083

Purpose

Expression of human p53 (1-73) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72P

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pCXLE-dCas9GFP-shP53

Plasmid

#102906

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9GFP-shP53

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV FLIP p53

Plasmid

#19745

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-p53 (Mouse 1-87)

Plasmid

#62061

Purpose

Expression of Mouse p53 (residues 1-87) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53 (Trp53 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-87

Promoter

T7

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pBSK-SN3 (wt p53)

Plasmid

#16421

Depositor

Bert Vogelstein

Article

Baker et al Science. 1989 Apr 14. 244(4901):217-21.

Insert

p53 (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
px330_P53 exon 8 gRNA

Plasmid

#68349

Purpose

px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.

Depositor

Jannik Elverløv-Jakobsen

Insert

P53 exon 8 gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
px330_P53 exon 7 gRNA

Plasmid

#68351

Purpose

px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.

Depositor

Jannik Elverløv-Jakobsen

Insert

P53 exon 7 gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK_hygro_p53(R280K)_V5

Plasmid

#136540

Purpose

Lentiviral expression vector for an inducible p53(R280K)-V5

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53(R280K) (TP53 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

V5

Expression

Mammalian

Mutation

p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
TP53RK gRNA (BRDN0001145338)

Plasmid

#77699

Purpose

3rd generation lentiviral gRNA plasmid targeting human TP53RK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TP53RK (TP53RK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TP53RK gRNA (BRDN0001146963)

Plasmid

#77700

Purpose

3rd generation lentiviral gRNA plasmid targeting human TP53RK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TP53RK (TP53RK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TP53RK gRNA (BRDN0001146381)

Plasmid

#77701

Purpose

3rd generation lentiviral gRNA plasmid targeting human TP53RK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TP53RK (TP53RK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pCSP1-mp53AS-6xMUT

Plasmid

#20904

Depositor

Stefano Piccolo

Article

Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

Insert

p53AS (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

point mutation (Ser/Thr-Ala) of the 6 N-terminal …

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pET28-p53 (Dog 1-77)

Plasmid

#62060

Purpose

Expression of Dog p53 (residues 1-77) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-77

Promoter

T7

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Mix.2-lux M-p53BE

Plasmid

#20894

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 M-p53BE promoter luciferase

Use

Tags

Expression

Mammalian

Mutation

point mutation of the p53 binding element (p53BE)…

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-N-terminus

Plasmid

#64677

Purpose

expresses only N terminal o WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Only N terminus amino acids 1-160

Promoter

CMV

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-C-terminus

Plasmid

#64679

Purpose

expresses only C terminus of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

only C terminus amino acids 500-548

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P27A)

Plasmid

#62068

Purpose

Expression of human p53 (1-73) (P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P27A

Promoter

T7

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only