We narrowed to 31,881 results for: Eng

1. pCA24N-ligase-cTF

   Plasmid

   #87743

   Purpose

   IPTG-inducible expression of ligase-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis and cTF (chimeric transcription factor base…

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

   Plasmid

   #169548

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbE1k-3

   Plasmid

   #71722

   Purpose

   tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

   Depositor

   Taek Soon Lee

   Article

   Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

   Inserts

   dihydropterine reductase (QDPR Human)
   pterin-4a-carbinolamine dehydratase (PCBD1 Human)
   

   Promoter

   pTrc

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB3K3 containing mRFP1

   Plasmid

   #118083

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, mRFP1
   

   Use

   Escherichia coli

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB1K3 containing mRFP1

   Plasmid

   #118082

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, mRFP1
   

   Use

   Escherichia coli

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK_P-GAP-ACC1*

   Plasmid

   #126740

   Purpose

   yeast plasmid overexpressing ACC1 from Komagataella phaffii

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acc1*
   

   Expression

   Yeast

   Mutation

   T3452G

   Promoter

   GAP

   Available Since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. asPink chromoprotein promoter-less

   Plasmid

   #118005

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, asPink
   

   Use

   Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p168

   Plasmid

   #62618

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZ_lacO-cP-AOX1-NpgA

    Plasmid

    #126731

    Purpose

    yeast plasmid overexpressing NpgA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    npgA
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    G951A,C957A

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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