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pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds

Plasmid

#119059

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity)

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Expression

Bacterial

Promoter

E1b minimal promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM6-N-22

Plasmid

#54172

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-PMP-N-10

Plasmid

#55439

Purpose

Localization: Peroxisomes, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PMP (PXMP2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Vinculin-23

Plasmid

#55453

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vinculin (VCL Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Rab4a-7

Plasmid

#55443

Purpose

Localization: Ras GTPase, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Rab4a (RAB4A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-Actin-7

Plasmid

#55462

Purpose

Localization: Actin, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Actin (ACTB Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mKO-Golgi-7

Plasmid

#57843

Purpose

Localization: Golgi Complex, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Golgi (B4GALT1 Human)

Tags

mKO

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDM3193

Plasmid

#113390

Purpose

Alpha factor inducible Ty3 with his3AI

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3-his3AI

Tags

his3AI

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Supervillin-45

Plasmid

#55136

Purpose

Localization: Membrane/Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Supervillin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
YPet-EB3-7

Plasmid

#56634

Purpose

Localization: MT End Binding Protein, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

EB3 (MAPRE3 Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pVRC-N-Venus-V150A-apCC-Di

Plasmid

#190706

Purpose

Encodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A-apCC-Di

Mutation

V150A

Promoter

araBAD

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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BII-C3H

Plasmid

#133386

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

Plasmid

#119070

Purpose

Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled1-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

Plasmid

#119071

Purpose

Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled2-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS427-pCSDest2-AcrE2

Plasmid

#85677

Purpose

Mammalian expression of anti-CRISPR protein from Type I-E

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type I-E anti-CRISPR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Myotilin-C-14

Plasmid

#55434

Purpose

Localization: Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Myotilin (MYOT Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only