We narrowed to 7,309 results for: aav

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AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-HcKCR1-EYFP

Plasmid

#182021

Purpose

Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

Depositor

Mingshan Xue

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR1

Use

AAV

Tags

EYFP

Expression

Mammalian

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-EGFP-NLS

Plasmid

#165441

Purpose

Expresses nuclear EGFP from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

EGFP-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-U6/sgClta-sfGFP-Clta

Plasmid

#200261

Purpose

U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

Depositor

Justin Eyquem

Article

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta

Use

AAV

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Txnip(C247S)

Plasmid

#206353

Purpose

AAV plasmid expressing mutant TXNIP

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C247S

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-CAG-mNG2(1-10)

Plasmid

#206042

Purpose

Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette

Use

CRISPR and Synthetic Biology; Donor template

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-4mtGCaMP6f-DIO

Plasmid

#179529

Purpose

Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Insert

4mtGCaMP6f

Use

AAV

Tags

4x Cox8 targeting sequence

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Article

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SypHer2-DAAO-NES

Plasmid

#119184

Purpose

Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

SypHer2-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC476 - pAAV c-fos eYFP

Plasmid

#47907

Purpose

An AAV vector that expresses eYFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

enhanced Yellow Fluorescent Protein

Use

AAV

Promoter

c-fos

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NFIA+SOX9

Plasmid

#129455

Purpose

Inducible overexpression of NFIA and Sox9 in human cells.

Depositor

Su-Chun Zhang

Article

Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

Insert

Sox 9 (SOX9 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-hM4D(Gi)-mCherry

Plasmid

#50479

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

Depositor

Bryan Roth

Has Service

AAV5

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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