We narrowed to 29,405 results for: des.2

1. PX459v2-ILK-gRNA 2-exon 8

   Plasmid

   #163321

   Purpose

   SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ILK gRNA 2_Exon8
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6F

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

2. pPGK-T7/2-CD44v8-10 (-cyt)

   Plasmid

   #137822

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v8-10 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LSB-hsa-let-7f-2-3p

   Plasmid

   #103155

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-let-7f-2-3p target (MIRLET7F2 Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-SARS-CoV-2-S-ectodomain

   Plasmid

   #235984

   Purpose

   Internally tagged SARS-CoV-2 spike ecto-domain

   Depositor

   Syed Saif Hasan

   Article

   Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

   Insert

   Spike protein (S SARS-CoV-2)
   

   Tags

   8xHis and Twin-Strep tag and internal Twin-Strep …

   Expression

   Mammalian

   Mutation

   aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(ZNF398_5-2)-PGKpuroBFP-W

   Plasmid

   #211998

   Purpose

   Express gRNA against ZNF398 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting ZNF398 (ZNF398 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(AAVS1-2)-PGKpuroBFP-W

   Plasmid

   #211955

   Purpose

   Express safe-cutter control gRNA with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   safe-cutter control sgRNA_AAVS1-2 (AAVS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-Teton-puro-shFDPS #2

   Plasmid

   #198760

   Purpose

   conditional knockdown of FDPS

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

   Insert

   shFDPS #2 (FDPS Human)
   

   Expression

   Mammalian

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. RPS4X sgRNA-2 lentiCRISPR v2 plasmid

   Plasmid

   #192232

   Purpose

   lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-OSF-ATG13 (2-197)-K18D

   Plasmid

   #188096

   Purpose

   Expresses human ATG13 HORMA K18D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-K18D

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-OSF-ATG13 (2-197)-K15D

    Plasmid

    #188094

    Purpose

    Expresses human ATG13 HORMA K15D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-ATG13 (2-197)-W50D

    Plasmid

    #188098

    Purpose

    Expresses human ATG13 HORMA W50D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-W50D

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-OSF-ATG13 (2-197)-Y152D

    Plasmid

    #188103

    Purpose

    Expresses human ATG13 HORMA Y152D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-Y152D

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-OSF-ATG13 (2-197)-R153D

    Plasmid

    #188104

    Purpose

    Expresses human ATG13 HORMA R153D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-R153D

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-OSF-ATG13 (2-197)-F16D

    Plasmid

    #188095

    Purpose

    Expresses human ATG13 HORMA F16D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-F16D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-OSF-ATG13 (2-197)-Y118D

    Plasmid

    #188101

    Purpose

    Expresses human ATG13 HORMA Y118D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-Y118D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-OSF-ATG13 (2-197)-E83L

    Plasmid

    #188099

    Purpose

    Expresses human ATG13 HORMA E83L mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-E83L

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-OSF-ATG13 (2-197)-Y115D

    Plasmid

    #188100

    Purpose

    Expresses human ATG13 HORMA Y115D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-Y115D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-OSF-ATG13 (2-197)-F19D

    Plasmid

    #188097

    Purpose

    Expresses human ATG13 HORMA F19D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-F19D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-OSF-ATG13 (2-197)-L151D

    Plasmid

    #188102

    Purpose

    Expresses human ATG13 HORMA L151D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-L151D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

    Plasmid

    #173891

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; gcry1: BFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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