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pH-nCas9-PPE-V2

Plasmid

#170131

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1, OsU3

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pUBC_V5_MS2-mCherry

Plasmid

#68420

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

V5 and mCherry

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

human UbC

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSCV.IRES.hCD4

Plasmid

#35712

Depositor

Patricia Ernst

Publication

Artinger et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Inserts

IRES
hCD4

Use

Retroviral

Expression

Mammalian

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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R5 EMCV

Plasmid

#51733

Purpose

Dicistronic vector with EMCV IRES

Depositor

Vincent Mauro

Publication

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

EMCV IRES

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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myc-hAtg13

Plasmid

#31965

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pGEX-4T1 human PP2A Aα full length

Plasmid

#132634

Purpose

expresses human PP2A Aα full length in E. coli

Depositor

Wenqing Xu

Publication

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A Aα (PPP2R1A Human)

Expression

Bacterial

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mNaChBacMut-T2A-tdTomato

Plasmid

#60657

Purpose

Expresses mNaChBacMut-T2A-tdTomato

Depositor

Massimo Scanziani

Publication

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBacMut-T2A-tdTomato

Expression

Mammalian

Mutation

E191K

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pDuRCC-K

Plasmid

#81193

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Publication

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below., Please view…

Promoter

ROX3 and SNP52p

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor_AcCAST_kanR

Plasmid

#127925

Purpose

Kanamycin resistance donor for AcCAST

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-c4X

Plasmid

#75288

Purpose

Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

Depositor

Paul Riggs

Publication

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available since

July 19, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pCXN2KS-Dsup

Plasmid

#90019

Purpose

To establish stable line expressing Dsup in constitutive manner

Depositor

Takekazu Kunieda

Publication

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Expression

Mammalian

Promoter

CAG

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137139

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP

Plasmid

#137153

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

W179F

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-iC++-EYFP

Plasmid

#137157

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP

Plasmid

#137140

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRK-flag-GFP11

Plasmid

#78590

Purpose

Expression flag-GFP 11 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 11

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAC-Luc

Plasmid

#33154

Depositor

Michael Rosbash

Publication

Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Insect

Promoter

Actin 5C

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only