We narrowed to 2,514 results for: organization Addgene

1. pEGFP_E1770Q-talin1 (1-2541)

   Plasmid

   #166121

   Purpose

   For expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   E1770Q-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E1770Q

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET_RGG-RFP-RGG

   Plasmid

   #124944

   Purpose

   Exoression of RGG-RFP-RGG

   Depositor

   Benjamin Schuster, Matthew Good, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   His and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZJOM12

   Plasmid

   #133638

   Purpose

   Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFE-vcDAB

   Plasmid

   #133004

   Purpose

   pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   vcdabB
   vcdabA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVenus-N1 cyclin B1 L45A (1324)

   Plasmid

   #61851

   Purpose

   mammalian expression of cyclin B1 L45 A mutant fused to Venus

   Depositor

   Jonathon Pines

   Article

   Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

   Insert

   cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   L45A

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

6. ZJOM8

   Plasmid

   #133671

   Purpose

   Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SNF7-GFP (SNF7 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLaNGo11-3

   Plasmid

   #126054

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11x3 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMiNGo11-1

   Plasmid

   #126056

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-GFP (F64L, S65T, V163A)-His3MX6

   Plasmid

   #53185

   Purpose

   chromosomal tagging with stable GFP

   Depositor

   John Pringle

   Article

   Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

   Insert

   HIs3MX6 (HIS3 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (F64L, S65T, V163A)

   Expression

   Mutation

   Promoter

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pTwist-iS-methadoneSnFR-Golgi

    Plasmid

    #220865

    Purpose

    Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR

    Depositor

    Henry Lester

    Article

    Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

    Insert

    iS-methadoneSnFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHHM.X513.iS-methadoneSnFR

    Plasmid

    #220861

    Purpose

    Bacterial expression of iS-methadoneSnFR for purification and in vitro assays.

    Depositor

    Henry Lester

    Article

    Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

    Insert

    iS-methadoneSnFR
    

    Use

    Tags

    His6-HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)

    Plasmid

    #233542

    Purpose

    A CMV promoter driving expression of a murine dominant negative myc tagged TCF4.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF4 (TCF4 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    lacks aa 1-56

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE60 G121A wgMDH

    Plasmid

    #204252

    Purpose

    Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G121A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE60 R196A wgMDH

    Plasmid

    #204271

    Purpose

    Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE60 R196E wgMDH

    Plasmid

    #204272

    Purpose

    Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196E

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE60 P123A wgMDH

    Plasmid

    #204254

    Purpose

    Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P123A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE60 R130Q wgMDH

    Plasmid

    #204268

    Purpose

    Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130Q

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE60 G127F wgMDH

    Plasmid

    #204264

    Purpose

    Bacterial expression of G127F wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G127F

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE60 P126R wgMDH

    Plasmid

    #204261

    Purpose

    Bacterial expression of P126R wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P126R

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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