We narrowed to 2,414 results for: organization Addgene

1. Lenti-FAB(mito-LD)

   Plasmid

   #214421

   Purpose

   Detection of mito-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and RspAN-3XHA-V5

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-HOT-Clover-BlastR

   Plasmid

   #138569

   Purpose

   Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   Clover-BlastR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR_L1-Turbo-YFP-NLS-STOP-L2

   Plasmid

   #127361

   Purpose

   gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mApple-6xHp

   Plasmid

   #214410

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   Spastin aa 43-92 (SPAST Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SacI ML GFP Strand 11 Long

   Plasmid

   #164120

   Purpose

   Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Long
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SP-GFP-CD274-NU

   Plasmid

   #121139

   Purpose

   Mammalian expression of GFP tagged CD274

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Inserts

   CD274 (CD274 Human)
   CD274 (CD274 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-FAB(ER-LD)

   Plasmid

   #214420

   Purpose

   Detection of ER-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and RspAN-FRB-V5

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR_L1-YFP-miniTurbo-NES-L2

   Plasmid

   #127351

   Purpose

   gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FAB(PX-LD)

   Plasmid

   #214426

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   FKBP-V5
   

   Use

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA_RGG-GFP-RGG_withTEVsite

    Plasmid

    #124939

    Purpose

    Expresion of RGG_GFP-RGG

    Depositor

    Daniel Hammer, Benjamin Schuster, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    TEV site between RGG and EGFP

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-NPC1(P691S)-FLAG

    Plasmid

    #164973

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P691S

    Promoter

    EF1a

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

    Plasmid

    #198337

    Purpose

    PB plasmid expressing Halo-tagged PP7 coat protein

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    PP7 coat protein 3x Halo-GB1
    

    Use

    Piggybac

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-NPC1(F503/4A)-FLAG

    Plasmid

    #164974

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F503A and F504A

    Promoter

    EF1a

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZJOM43

    Plasmid

    #133652

    Purpose

    Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPS4-DuDre (VPS4 Budding Yeast)
    

    Use

    Tags

    DuDre

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SacI ML GFP Strand 11 Short

    Plasmid

    #164121

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    SacI ML GFP Strand 11 Short
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB CAG Otx2-IRES-EGFP

    Plasmid

    #153947

    Purpose

    piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    Otx2 (Otx2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid

    #127349

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

    Plasmid

    #127362

    Purpose

    gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET_SZ1-RGG-GFP-RGG

    Plasmid

    #124937

    Purpose

    Expression of SZ1-RGG-GFP-RGG

    Depositor

    Daniel Hammer, Benjamin Schuster, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge