We narrowed to 5,818 results for: pCas

1. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

   Plasmid

   #128171

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-AaCas12b(R911A)-2AeGFP

   Plasmid

   #121961

   Purpose

   Mammalian expression, Transcription regulation, deactivated AaCas12b

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   Catalytically deactivated AaCas12b
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   R911A

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PX544 SpCas9(del1098-1368)

   Plasmid

   #58889

   Purpose

   Truncated form of SpCas9 without C' domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 1098-1368

   Available Since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-dHeFSpCas9-VP64-6xHis

   Plasmid

   #92119

   Purpose

   Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   T7

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-PB1-MontiRed-LOV2

   Plasmid

   #178872

   Purpose

   Mammalian expression of PB1-MontiRed-LOV2

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-MontiRed-LOV2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAWp63-clone32 (Opti-SpCas9)

   Plasmid

   #131736

   Purpose

   Expresses Opti-SpCas9 in mammalian cells

   Depositor

   Alan Siu Lun Wong

   Article

   Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

   Insert

   Opti-SpCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   R661A+K1003H

   Promoter

   EFS

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Osa-pCAMBIA-1301-STTM394

   Plasmid

   #84204

   Purpose

   Disturb the expression of miR394 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR394
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCASP-SptP120-E3_5-HilA

   Plasmid

   #153326

   Purpose

   Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-E3_5 DARPin
   

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98976

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(N863A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCASP-SptP120-K27-HilA

    Plasmid

    #153328

    Purpose

    Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K27 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCA-ATG-intron-tdT3Myc

    Plasmid

    #36883

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tdTomato-3Myc
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    beta-globin intron containing a loxP site inserte…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-RFP-miR-E1-1int

    Plasmid

    #19823

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    miRNA
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pBW809_pCAG-PV6-F3-mCherry-BGHpA

    Plasmid

    #87585

    Purpose

    PV6 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Osa-pCAMBIA-1301-STTM166

    Plasmid

    #84197

    Purpose

    Disturb the expression of miR166 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR166
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS/Daxx myc S669A

    Plasmid

    #19282

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    Daxx myc S669A (Daxx Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pBW820_pCAG-pExpr-FULL-ADDER

    Plasmid

    #87552

    Purpose

    Encodes full adder logic in response to Cre, Flp and VCre recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP, mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCaSpeR4 cyto Grx1-roGFP2

    Plasmid

    #64999

    Purpose

    Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    roGFP2

    Expression

    Insect

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-PB1-MontiRed-LOV2dark

    Plasmid

    #178874

    Purpose

    Mammalian expression of PB1-MontiRed-LOV2dark

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-MontiRed-LOV2dark
    

    Expression

    Mammalian

    Mutation

    LOV2: C450A, L514K, G528A, L531E, N538E

    Promoter

    CAG

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge