59,458 results

1. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available Since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. NEK7_HUMAN_D0

   Plasmid

   #79735

   Purpose

   This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   NEK7 (NEK7 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. TFORF2043

   Plasmid

   #142316

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARID3A (ARID3A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG PTEN

   Plasmid

   #78777

   Purpose

   To overexpress PTEN in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   PTEN (PTEN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM

   Plasmid

   #71250

   Purpose

   luciferase reporter with human GM-CSF promoter -627 to + 28

   Depositor

   Peter Cockerill

   Article

   Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

   Insert

   GM-CSF promoter -627 to +28 fragment (CSF2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   GM-CSF

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-CMV-hXRCC1-mCherry-Hygro

   Plasmid

   #176532

   Purpose

   mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

   Insert

   X-Ray Repair Cross Complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEM705 Gsi (Cys)

   Plasmid

   #109373

   Purpose

   GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsi chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Cys at position 391 (Pertussis Toxin Sensitive)

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR1316

   Plasmid

   #111126

   Purpose

   Expresses FANCA.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCA (FANCA Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CCR5_gRNA_pX459

    Plasmid

    #192268

    Purpose

    gRNA expression for CRISPR Genome Editing of CCR5

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    CCR5 guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TERTpWT_Gluc_CCR5

    Plasmid

    #192270

    Purpose

    Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    Promoter Reporter Insert within donor template
    

    Use

    CRISPR

    Promoter

    TERT promoter driving expression of gaussia

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. RC(WW)-LgBit

    Plasmid

    #208931

    Purpose

    Recognition domain for in vitro MAPK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    WW domain of PIN1 (PIN1 Human)
    

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Rab27A

    Plasmid

    #89237

    Purpose

    Expression of GFP-tagged human Rab27A in mammalian cells

    Depositor

    William Gahl

    Article

    Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

    Insert

    human Rab27A (RAB7A Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. LRP6 deltaN-pCS2-VSVG

    Plasmid

    #27281

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Mutation

    contains amino acid residues 1370–1613 (extracell…

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-UBXD4-Strep-HA

    Plasmid

    #113480

    Purpose

    Expression of human UBXD4 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD4 (UBXN2A Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLIK MEK5-DD-mRFP1 neo

    Plasmid

    #47548

    Purpose

    Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    MEK5-DD-mRFP1 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S311D, T315D

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pMJA089

    Plasmid

    #128518

    Purpose

    Expresses human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme (LYZ Human)
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE-UBC-hCD47-T2A-GFP

    Plasmid

    #205458

    Purpose

    lentiviral plasmid for expression of human CD47

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD47 (CD47 Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Nucleolin

    Plasmid

    #28176

    Depositor

    Michael Kastan

    Article

    Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

    Insert

    Nucleolin (NCL Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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