We narrowed to 5,097 results for: puc
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Plasmid#162998Purposeplasmid expressing ABE with dCas9DepositorInsertdABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15002-ABEmax
Plasmid#163006Purposeplasmid expressing ABEmaxDepositorInsertABEmax
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15064-eCBE
Plasmid#163009Purposeplasmid expressing eCBEDepositorInserteCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WF
Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAID5VY
Plasmid#165048PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ3
Plasmid#83558PurposeUsed to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.DepositorInsertGAL2 promoter-yEGFP
ExpressionYeastPromoterGAL2Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
S8_attL1-N-term-EGFP-attL2
Plasmid#128472PurposeEGFP entry clonesDepositorInsertEGFP entry clones
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW063
Plasmid#115950PurposeDestination VectorDepositorInsertLvl0 SGP (+/- Apt) Dest
UseSynthetic BiologyAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGC001
Plasmid#48824PurposeProvides WUS as GreenGate coding sequence module.DepositorInsertWUS (WUS Mustard Weed)
UseGolden gate compatible cloning vectorMutationinternal BsaI recognition site removed by silent …Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AP1297‐1
Plasmid#87247Purposegtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
Tagsgtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBL712
Plasmid#140382PurposeExpresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoterDepositorInsertT7-otrO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH71
Plasmid#128214Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAP1680
Plasmid#137088PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFPDepositorInserttagRFP
UsePcr template for donor dnaExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW322
Plasmid#115953PurposeDestination VectorDepositorInsertLvl1 P1 With 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW323
Plasmid#115954PurposeDestination VectorDepositorInsertLvl1 P1 No 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW325
Plasmid#115956PurposeDestination VectorDepositorInsertLvl1 P2 With 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW326
Plasmid#115957PurposeDestination VectorDepositorInsertLvl1 P2 No 3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW327
Plasmid#115958PurposeDestination VectorDepositorInsertLvl1 P2 End
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW328
Plasmid#115959PurposeDestination VectorDepositorInsertLvl1 P3 With E1-3'UTR
UseSynthetic BiologyAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pATM (pKD-G11)
Plasmid#62690PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP123/amiR-eGFP419/tdTomato
UseRNAiExpressionMammalianAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pADH118-17
Plasmid#91727PurposeNAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.DepositorInsertNAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
Available SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH143-2
Plasmid#90990PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH100-2
Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1044
Plasmid#63797PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection markerDepositorInsert3xFLAG followed by KlLEU2
UseE. coli cloning vectorTags3xFLAGPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKH032
Plasmid#224548Purpose2A-3xNLS-2xClover mouse Car2 C-term targeting vectorDepositorInsert2xClover
UseMouse TargetingAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKH040
Plasmid#224549Purpose2A-3xNLS-tdTomato mouse Car2 C-term targeting vectorDepositorInserttdTomato
UseCRISPR and Mouse TargetingAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-mRuby2-P2A-PuroR_pKG02755
Plasmid#246371PurposeIVT template for making modRNA encoding mRuby2-P2A-PuroRDepositorInsertmRuby2-P2A-PuroR
UseSynthetic Biology; In vitro transcriptionExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
CIRC-V1-Tet-CVB3 IRES-EGFP-BbsI
Plasmid#246756PurposeRNA circularization with CIRC using Tet intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.DepositorInsertCIRC-V1-Tet-CVB3 IRES-EGFP-BbsI
UseSynthetic BiologyAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
AGG1767 PUb-Bs-TRE-Bn
Plasmid#242441PurposeAe aegypti PUb promoter expressing barstar, and TRE expressing barnaseDepositorInsertsBarstar
DsRED
barnase
ExpressionInsectPromoterAePUb, Hr5/IE1, and TREAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
apoBb.1-Dendra2
Plasmid#218180Purposeused for TALEN HDR to generate endogenous fusion of HA-Dendra2 to apoBb.1 in zebrafishDepositorInsertapoBb.1-Dendra2
UseTALENTagsfuses HA-Dendra2 to apoBb.1Mutationinserts HA-Dendra2 sequence at C-terminus of apoB…Available SinceAug. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1376
Plasmid#239829PurposeWT OrufIscB omegaRNA mammalian expressionDepositorInsertOrufIscB omegaRNA scaffold
ExpressionMammalianPromoterhuman U6Available SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPZ1A9
Plasmid#223835PurposeExpressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiaeDepositorInsertpTEF1>mTagBFP>mNeonGreen>
ExpressionYeastAvailable SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13A7
Plasmid#223837PurposeExpressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiaeDepositorInsertpTEF1>mScarletI3>
ExpressionYeastPromoterTEF1Available SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only