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We narrowed to 9,302 results for: BLI;

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Showing: 4501 - 4550 of 9302 results

  1. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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  2. KG#865

    Plasmid
    #110912
    Purpose
    Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers
    Depositor
    Kenneth Miller
    Insert
    Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
    Expression
    Bacterial
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110912-plasmid-map-sequence-id-212549

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  3. KG#883

    Plasmid
    #110913
    Purpose
    Coding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a protein
    Depositor
    Kenneth Miller
    Insert
    5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
    Expression
    Bacterial
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110913-plasmid-map-sequence-id-212540

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  4. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

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  5. pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53205
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and kanMX6
    Promoter
    Gal1 promoter
    Available Since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid
    #180157
    Purpose
    td-sfGFP dre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Mouse Targeting; Dre/rox
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180157-plasmid-map-sequence-id-355323

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  7. PTPN5

    Plasmid
    #38887
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C496S inactivating mutation
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. RHOU

    Plasmid
    #38885
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOU (RHOU Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C191L
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pFLAG-6a-Ataxin3Q22-UIM*

    Plasmid
    #22128
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UIM 1,2,3 SA-DG
    Available Since
    Nov. 17, 2009
    Availability
    Academic Institutions and Nonprofits only

  10. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pT7-7 asyn S87A/S129E

    Plasmid
    #36065
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87A/S129E (SNCA Human)
    Expression
    Bacterial
    Mutation
    S87A/S129E
    Promoter
    T7
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36065-plasmid-map-sequence-id-44522

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  12. pVP16-tev-PRT1

    Plasmid
    #83405
    Purpose
    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83405-plasmid-map-sequence-id-175081

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  13. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  14. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

    Enlarge

  15. SDCBPA-c010

    Plasmid
    #175482
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175482-plasmid-map-sequence-id-353168

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  16. pNtCp-rbcL-PHLS-aadA-accD

    Plasmid
    #52470
    Purpose
    Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum
    Depositor
    Anastasios Melis
    Insert
    rbcL-PHLS-aadA-accD
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52470-plasmid-map-sequence-id-81333

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  17. pLV-Enh eGFP Reporter-muKC2-IGR13

    Plasmid
    #59282
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    17kb upstream krt5
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. PTGR2

    Plasmid
    #38949
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTGR2 (PTGR2 Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. mEos4a-CenpA-C-22

    Plasmid
    #62772
    Purpose
    Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    CenpA (CENPA Human)
    Tags
    mEos4a
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. FABP9

    Plasmid
    #39107
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FABP9 (FABP9 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. pCB99

    Plasmid
    #69745
    Purpose
    Retroviral expression of Centrin 2
    Depositor
    Iain Cheeseman
    Insert
    Centrin 2 (CETN2 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. 4QQN

    Plasmid
    #61951
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HRMT1L3:APC035-H01:C21507 (PRMT3 Human)
    Tags
    6xHis-Thrombin and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. AKR7A3

    Plasmid
    #38862
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKR7A3 (Akr7a3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Tags
    NusA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74295-plasmid-map-sequence-id-141881

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  26. 4JDS

    Plasmid
    #60081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD7:DCC011-H01:C212434 (SETD7 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. pDK713

    Plasmid
    #134229
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK360 tdTomato (long SKAP) (KNSTRN Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Available Since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134229-plasmid-map-sequence-id-272275

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  28. pCRII-Topo Couptf1

    Plasmid
    #45870
    Depositor
    Jeffrey Macklis
    Insert
    Couptf1 in situ probe (Nr2f1 Mouse)
    Use
    In situ
    Mutation
    contains bp# 1755-2381 of NM_010151.2
    Available Since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. PRKCG

    Plasmid
    #38938
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRKCG (PRKCG Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

    Enlarge

  31. VIPR2

    Plasmid
    #39044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    VIPR2 (VIPR2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. Myc-Foxo1 LAALL (pCMV5)

    Plasmid
    #17548
    Depositor
    Domenico Accili
    Insert
    Foxo1 LAALL (Foxo1 Mouse)
    Use
    RNAi
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    LAALL (K460A; E461A)
    Promoter
    CMV
    Available Since
    Oct. 8, 2008
    Availability
    Academic Institutions and Nonprofits only

  33. mEos4a-CenpB-N-22

    Plasmid
    #62773
    Purpose
    Localization: Nucleus/Centromeres, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    CenpB (CENPB Human)
    Tags
    mEos4a
    Expression
    Mammalian
    Mutation
    Encodes amino acids 1-180
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. 4QQ4

    Plasmid
    #61945
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MORC3:JMC01B-H03:C203041 (MORC3 Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. CS2-H2B-EGFP

    Plasmid
    #132414
    Purpose
    To make synthetic mRNA encoding a nuclear EGFP
    Depositor
    David Kimelman
    Insert
    H2B-EGFP (H2BC21 Synthetic)
    Use
    Synthetic mrna production
    Promoter
    SP6
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    132414-plasmid-map-sequence-id-257700

    Enlarge

  36. pLV-Enh eGFP Reporter-muKC2-X39

    Plasmid
    #59263
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt83-ex5-7
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pZC10 (pFA6a-PSc-6xGly-V5-His3MX)

    Plasmid
    #44067
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44067-plasmid-map-sequence-id-61693

    Enlarge

  39. pCRII-Topo Hnrp

    Plasmid
    #45872
    Depositor
    Jeffrey Macklis
    Insert
    Hnrnpa2b1 in situ probe (Hnrpa2b1 Mouse)
    Use
    In situ
    Mutation
    contains bp# 1145-1370 of NM_182650.3
    Available Since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

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  41. pAc5.1B-EGFP-DmMTR-4

    Plasmid
    #66591
    Purpose
    expresses EGFP-tagged MTR-4 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    MTR-4 (l(2)35Df Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Promoter
    Actin 5C
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66591-plasmid-map-sequence-id-113866

    Enlarge

  42. pLV-Enh eGFP Reporter-muKC2-X33

    Plasmid
    #59257
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt81-ex4-7
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53207
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and HIS3MX6
    Promoter
    GAL1 promoter
    Available Since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pCB30

    Plasmid
    #111910
    Purpose
    Expresses gRNA to target URA3 knockout locus and has KanMX marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting URA3 knockout locus
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    SNR52p
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111910-plasmid-map-sequence-id-218310

    Enlarge

  45. MYLK4

    Plasmid
    #39175
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MYLK4 (MYLK4 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Mutation
    N371S, codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  46. pHT-PRD

    Plasmid
    #87755
    Purpose
    Bacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)
    Depositor
    Yu-Wen Hwang
    Insert
    Semi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
    Tags
    6X histidine tag
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase promoter
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87755-plasmid-map-sequence-id-173677

    Enlarge

  47. RAC3

    Plasmid
    #38821
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RAC3 (rac3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pLV-Enh eGFP Reporter-muKC2-X14

    Plasmid
    #59238
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt8-ex5-8
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. pLV-Enh eGFP Reporter-muKC2-IGR12

    Plasmid
    #59281
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    1.5kb upstream krt5
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. HDHD1A

    Plasmid
    #39039
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HDHD1A (PUDP Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    G102R
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4501 - 4550 of 9302 results